Borrelia burgdorferi binds fibronectin through a tandem β-zipper, a common mechanism of fibronectin binding in staphylococci, streptococci, and spirochetes

Sophie Raibaud, Ulrich Schwarz-Linek, Jung Hwa Kim, Huw T. Jenkins, Elizabeth R. Baines, Sivashankarappa Gurasiddappa, Magnus Höök, Jennifer R. Potts

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

BBK32 is a fibronectin-binding protein from, the Lyme disease-causing spirochete Borrelia burgdorferi. In this study, we show that BBK32 shares sequence similarity with fibronectin module-binding motifs previously identified in proteins from Streptococcus pyogenes and Staphylococcus aureus. Nuclear magnetic resonance spectroscopy and isothermal titration calorimetry are used to confirm the binding sites of BBK32 peptides within the N-terminal domain of fibronectin and to measure the affinities of the interactions. Comparison of chemical shift perturbations in fibronectin F1 modules on binding of peptides from BBK32, FnBPA from S. aureus, and SfbI from S. pyogenes provides further evidence for a shared mechanism of binding. Despite the different locations of the bacterial attachment sites in BBK32 compared with SfbI from S. pyogenes and FnBPA from S. aureus, an antiparallel orientation is observed for binding of the N-terminal domain of fibronectin to each of the pathogens. Thus, these phylogenetically and morphologically distinct bacterial pathogens have similar mechanisms for binding to human fibronectin.

Original languageEnglish (US)
Pages (from-to)18803-18809
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number19
DOIs
StatePublished - May 13 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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