Bone marrow transplantation platform to investigate the role of dendritic cells in graft-versus-host disease

Hung D. Nguyen, Phung Thanh Huong, Krystal Hossack, Sanjeev Gurshaney, Kevin Ezhakunnel, Thien Huong Huynh, Anamaria Morales Alvarez, Nhat Tu Le, Hung N. Luu

Research output: Contribution to journalArticle

Abstract

Allogeneic bone marrow transplantation (BMT) is an effective therapy for hematological malignancies due to the graft-versus-leukemia (GVL) effect to eradicate tumors. However, its application is limited by the development of graft-versus-host disease (GVHD), a major complication of BMT. GVHD is evoked when T-cells in the donor grafts recognizealloantigen expressed by recipient cells and mount unwanted immunological attacks against recipient healthy tissues. Thus, traditional therapies are designed to suppress donor T-cell alloreactivity. However, these approaches substantially impair the GVL effect so that the recipient's survival is not improved. Understanding the effects of therapeutic approaches on BMT, GVL, and GVHD, is thus essential. Due to the antigen-presenting and cytokine-secreting capacities to stimulate donor T-cells, recipient dendritic cells (DCs) play a significant role in the induction of GVHD. Therefore, targeting recipient DCs becomes a potential approach for controlling GVHD. This work provides a description of a novel BMT platform to investigate how host DCs regulate GVH and GVL responses after transplantation. Also presented is an effective BMT model to study the biology of GVHD and GVL after transplantation.

Original languageEnglish (US)
Article numbere60083
JournalJournal of Visualized Experiments
Volume2020
Issue number157
DOIs
StatePublished - Mar 2020

Keywords

  • Bone marrow transplantation
  • Dendritic cell co-transplantation
  • Dendritic cells
  • Graft-versus-host disease
  • Graft-versus-leukemia
  • Hematopoietic stem cell transplantation
  • Immunology and Infection
  • Issue 157

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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