Block-Cell-Printing for live single-cell printing

Kai Zhang, Chao Kai Chou, Xiaofeng Xia, Mien Chie Hung, Lidong Qin

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

A unique live-cell printing technique, termed "Block-Cell- Printing" (BloC-Printing), allows for convenient, precise, multiplexed, and high-throughput printing of functional single-cell arrays. Adapted from woodblock printing techniques, the approach employs microfluidic arrays of hook-shaped traps to hold cells at designated positions and directly transfer the anchored cells onto various substrates. BloC-Printing has a minimum turnaround time of 0.5 h, a maximum resolution of 5 μm, close to 100% cell viability, the ability to handle multiple cell types, and efficiently construct protrusion-connected single-cell arrays. The approach enables the large-scale formation of heterotypic cell pairs with controlled morphology and allows for material transport through gap junction intercellular communication. When six types of breast cancer cells are allowed to extend membrane protrusions in the BloC-Printing device for 3 h, multiple biophysical characteristics of cells - including the protrusion percentage, extension rate, and cell length - are easily quantified and found to correlate well with their migration levels. In light of this discovery, BloC-Printing may serve as a rapid and high-throughput cell protrusion characterization tool to measure the invasion and migration capability of cancer cells. Furthermore, primary neurons are also compatible with BloC-Printing.

Original languageEnglish (US)
Pages (from-to)2948-2953
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number8
DOIs
StatePublished - Feb 25 2014

Keywords

  • Cell array
  • Cell communication
  • Neuron patterning
  • Protrusion profiling

ASJC Scopus subject areas

  • General

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