Bis-(2-ethylexhyl) phthalate impairs spermatogenesis in zebrafish (Danio rerio)

Bruna Corradetti, Alessandra Stronati, Luca Tosti, Giancarlo Manicardi, Oliana Carnevali, Davide Bizzaro

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


Bis-(2-ethylhexyl) phthalate (DEHP) is a widely used industrial additive for increasing plastic flexibility. Its metabolites are known to exert toxic effects on reproduction and development of mammals. The aim of this study was to evaluate the effects of environmentally relevant concentrations of DEHP (0.2 and 20. μg/L) on the reproductive biology of adult male zebrafish ( Danio rerio). The effects of DEHP and 17β-ethynylestradiol (a positive control) were determined after one or three weeks of exposure by TUNEL assay, histomorphometric analysis and evaluation of reproductive performance. DEHP impaired reproduction in zebrafish by inducing a mitotic arrest during spermatogenesis, increasing DNA fragmentation in sperm cells and markedly reducing embryo production (up to 90%). In conclusion, relatively short-term exposure to environmentally relevant concentrations of DEHP is able to alter spermatogenesis and affect reproduction in zebrafish.

Original languageEnglish (US)
Pages (from-to)195-202
Number of pages8
JournalReproductive Biology
Issue number3
StatePublished - Sep 2013


  • DEHP
  • Danio rerio
  • Endocrine disruptors
  • Spermatogenesis
  • Zebrafish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology
  • Developmental Biology


Dive into the research topics of 'Bis-(2-ethylexhyl) phthalate impairs spermatogenesis in zebrafish (Danio rerio)'. Together they form a unique fingerprint.

Cite this