TY - JOUR
T1 - Bis-(2-ethylexhyl) phthalate impairs spermatogenesis in zebrafish (Danio rerio)
AU - Corradetti, Bruna
AU - Stronati, Alessandra
AU - Tosti, Luca
AU - Manicardi, Giancarlo
AU - Carnevali, Oliana
AU - Bizzaro, Davide
PY - 2013/9
Y1 - 2013/9
N2 - Bis-(2-ethylhexyl) phthalate (DEHP) is a widely used industrial additive for increasing plastic flexibility. Its metabolites are known to exert toxic effects on reproduction and development of mammals. The aim of this study was to evaluate the effects of environmentally relevant concentrations of DEHP (0.2 and 20. μg/L) on the reproductive biology of adult male zebrafish ( Danio rerio). The effects of DEHP and 17β-ethynylestradiol (a positive control) were determined after one or three weeks of exposure by TUNEL assay, histomorphometric analysis and evaluation of reproductive performance. DEHP impaired reproduction in zebrafish by inducing a mitotic arrest during spermatogenesis, increasing DNA fragmentation in sperm cells and markedly reducing embryo production (up to 90%). In conclusion, relatively short-term exposure to environmentally relevant concentrations of DEHP is able to alter spermatogenesis and affect reproduction in zebrafish.
AB - Bis-(2-ethylhexyl) phthalate (DEHP) is a widely used industrial additive for increasing plastic flexibility. Its metabolites are known to exert toxic effects on reproduction and development of mammals. The aim of this study was to evaluate the effects of environmentally relevant concentrations of DEHP (0.2 and 20. μg/L) on the reproductive biology of adult male zebrafish ( Danio rerio). The effects of DEHP and 17β-ethynylestradiol (a positive control) were determined after one or three weeks of exposure by TUNEL assay, histomorphometric analysis and evaluation of reproductive performance. DEHP impaired reproduction in zebrafish by inducing a mitotic arrest during spermatogenesis, increasing DNA fragmentation in sperm cells and markedly reducing embryo production (up to 90%). In conclusion, relatively short-term exposure to environmentally relevant concentrations of DEHP is able to alter spermatogenesis and affect reproduction in zebrafish.
KW - DEHP
KW - Danio rerio
KW - Endocrine disruptors
KW - Spermatogenesis
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=84883501174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883501174&partnerID=8YFLogxK
U2 - 10.1016/j.repbio.2013.07.003
DO - 10.1016/j.repbio.2013.07.003
M3 - Article
C2 - 24011190
AN - SCOPUS:84883501174
SN - 1642-431X
VL - 13
SP - 195
EP - 202
JO - Reproductive Biology
JF - Reproductive Biology
IS - 3
ER -