Previous studies in this laboratory have shown that benzo(a)pyrene (BaP) modulates protein kinase C (PKC)‐mediated phosphorylation of aortic smooth muscle cell (SMC) proteins. This observation is consistent with the ability of other aromatic hydrocarbons (AHs), such as 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD), to modulate kinase activities in cells of hepatic, testicular, and thymic origin. Because all these chemicals share the ability to bind the aryl hydrocarbon receptor (AhR), the present studies were conducted to determine if changes in PKC activity by AHs conform with established structure‐activity relationships. Experiments were conducted to examine the effects of TCDD, 2,3,7,8‐tetrachlorodibenzofuran (TCDF), and 2,8‐dichlorodibenzodioxin (DCDD) on the phosphorylation of exogenous histone type‐III under basal and PKC‐activating conditions. These congeners exhibit both high (TCDD and TCDF) and low (DCDD) AhR agonist activities. Measurements of kinase activity were conducted in the cytosolic and particulate fractions of growth‐arrested (i.e., serum‐deprived) cultured rat aortic SMCs incubated with 10 nM TCDD, TCDF, and DCDD for 0.5, 12, or 24 hours. No changes in basal kinase activity were induced by these chemicals at any of the times tested. Significant decreases in cytosolic and particulate PKC activity relative to controls were observed upon exposure of SMCs for 0.5 hours to 10 nM TCDD, TCDF, and DCDD. In contrast, SMCs exposed to TCDD and TCDF for 12 hours exhibited a significant increase in PKC activity in both cytosolic and particulate fractions. The PKC activity in cells exposed to DCDD for 12 hours was not altered. Prolonged exposure of SMCs to 10 nM TCDD, TCDF, and DCDD for 24 hours decreased PKC activity in the cytosolic fraction, while only TCDD and TCDF decreased particulate PKC activity. These data show that PKC activity is modulated differentially as a function of time in SMCs exposed to TCDD and related compounds. Collectively, the patterns of histone phosphorylation induced by these chemicals in rat aortic SMCs suggest that modulation of C‐kinase activity involves both receptor‐independent and receptor‐related events.
- Rat Aortic Smooth Muscle Cells
ASJC Scopus subject areas