Biosynthesis of Two New Steroids in the Human Foetal Liver, 1 β‐ and 2β‐Hydroxytestosterone

B. P. Lisboa; J. Gustafsson

doi:10.1111/j.1432-1033.1968.tb00463.x

Biosynthesis of Two New Steroids in the Human Foetal Liver, 1 β‐ and 2β‐Hydroxytestosterone

B. P. Lisboa, J. Gustafsson

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Two new C₁₉‐steroids with a 3‐oxo‐Δ⁴‐structure have been isolated and identified after incubation of testosterone with 105,000 × g microsomes of human foetal liver. These two ultraviolet positive metabolites were isolated by ascending one‐dimensional thin‐layer chromatography. The isolated compounds were identified as 1β‐ and 2β‐hydroxytestosterone by means of gas chromatography‐mass spectrometry. They were formed in approximately the same amount, each one representing about 15 % of the amount of 6β‐hydroxytestosterone formed.

Original language	English (US)
Pages (from-to)	419-424
Number of pages	6
Journal	European Journal of Biochemistry
Volume	6
Issue number	3
DOIs	https://doi.org/10.1111/j.1432-1033.1968.tb00463.x
State	Published - Nov 1968

ASJC Scopus subject areas

Biochemistry

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10.1111/j.1432-1033.1968.tb00463.x

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year = "1968",

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AU - Gustafsson, J.

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Y1 - 1968/11

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AB - Two new C19‐steroids with a 3‐oxo‐Δ4‐structure have been isolated and identified after incubation of testosterone with 105,000 × g microsomes of human foetal liver. These two ultraviolet positive metabolites were isolated by ascending one‐dimensional thin‐layer chromatography. The isolated compounds were identified as 1β‐ and 2β‐hydroxytestosterone by means of gas chromatography‐mass spectrometry. They were formed in approximately the same amount, each one representing about 15 % of the amount of 6β‐hydroxytestosterone formed.

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Biosynthesis of Two New Steroids in the Human Foetal Liver, 1 β‐ and 2β‐Hydroxytestosterone

Abstract

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