Biosynthesis of heparin. Loss of C-5 hydrogen during conversion of d-glucuronic to l-iduronic acid residues

Ulf Lindahl, Ingvar Jacobsson, Magnus Hook, Gudrun Backstrom, David S. Feingold

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Microsomal fraction from mouse mastocytoma was incubated with UDP-N-acetylglucosamine and 3H-, 14C-labeled UDP-glucuronic acid. Enzymatic sulfation of the resulting heparin-precursor polysaccharide (by addition of 3′-phosphoadenylylsulfate) was accompanied by selective loss of 3H from C5, but not from C2 or C4, of the uronic acid residues. Analysis of the sulfated product showed C5-3H in the glucuronic acid but not in the iduronic acid component. The formation of l-iduronic acid residues by C5-epimerization of d-glucuronic acid units at the polymer level thus involves loss of hydrogen at C5.

Original languageEnglish
Pages (from-to)492-499
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume70
Issue number2
DOIs
StatePublished - May 17 1976

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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