Microsomal fraction from mouse mastocytoma was incubated with UDP-N-acetylglucosamine and 3H-, 14C-labeled UDP-glucuronic acid. Enzymatic sulfation of the resulting heparin-precursor polysaccharide (by addition of 3′-phosphoadenylylsulfate) was accompanied by selective loss of 3H from C5, but not from C2 or C4, of the uronic acid residues. Analysis of the sulfated product showed C5-3H in the glucuronic acid but not in the iduronic acid component. The formation of l-iduronic acid residues by C5-epimerization of d-glucuronic acid units at the polymer level thus involves loss of hydrogen at C5.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 17 1976|
ASJC Scopus subject areas
- Molecular Biology