Abstract
6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[14C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver - as well as the placenta - is able to form 6β-hydroxy-C19-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 555-563 |
| Number of pages | 9 |
| Journal | Steroids |
| Volume | 11 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1968 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology
- Pharmacology
- Clinical Biochemistry
- Organic Chemistry
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