Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes

Jan Åke Gustafsson, Belisario P. Lisboa

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[14C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver - as well as the placenta - is able to form 6β-hydroxy-C19-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.

Original languageEnglish (US)
Pages (from-to)555-563
Number of pages9
JournalSteroids
Volume11
Issue number4
DOIs
StatePublished - Apr 1968

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes'. Together they form a unique fingerprint.

Cite this