Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes

Jan Åke Gustafsson; Belisario P. Lisboa

doi:10.1016/S0039-128X(68)80068-6

Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes

Jan Åke Gustafsson, Belisario P. Lisboa

Houston Methodist

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[¹⁴C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver - as well as the placenta - is able to form 6β-hydroxy-C₁₉-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.

Original language	English (US)
Pages (from-to)	555-563
Number of pages	9
Journal	Steroids
Volume	11
Issue number	4
DOIs	https://doi.org/10.1016/S0039-128X(68)80068-6
State	Published - Apr 1968

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(68)80068-6

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title = "Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes",

abstract = "6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[14C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver - as well as the placenta - is able to form 6β-hydroxy-C19-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.",

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T1 - Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes

AU - Gustafsson, Jan Åke

AU - Lisboa, Belisario P.

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N2 - 6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[14C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver - as well as the placenta - is able to form 6β-hydroxy-C19-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.

AB - 6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[14C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver - as well as the placenta - is able to form 6β-hydroxy-C19-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.

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Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes

Abstract

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