Biostable ssDNA aptamers specific for Hodgkin lymphoma.

Parag Parekh, Sanchit Kamble, Nianxi Zhao, Zihua Zeng, Jianguo Wen, Bin Yuan, Youli Zu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

As a "chemical antibody", oligonucleotide aptamers can specifically bind to their target molecules. However, clinical potential of aptamers in disease diagnosis is not yet fully explored. Using a tumor cell-based selection protocol, we developed single-stranded DNA aptamers for Hodgkin lymphoma (HL) tumor cells. The aptamers specifically bound to HL cells with a high affinity, reaching maximal cell binding at 10 nM final concentration. Importantly, the aptamers were able to selectively detect HL cells and did not react to other tumor or blood cells in mixed samples, indicating that the aptamers can be used as a specific probe for in vitro analysis of HL cells. Moreover, due to the inherent properties of DNA, the aptamers were stable in human serum, suggesting potential for in vivo detection of HL tumor cells.

Original languageEnglish
Pages (from-to)14543-14557
Number of pages15
JournalSensors (Basel, Switzerland)
Volume13
Issue number11
DOIs
StatePublished - Oct 25 2013

Keywords

  • Aptamer
  • Biostability
  • Hodgkin lymphoma
  • Single-stranded DNA (ssDNA)
  • Tumor cell detection

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

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