TY - JOUR
T1 - Biophysical characterization of VEGF-aHt DNA aptamer interactions
AU - Kanakaraj, Indhu
AU - Chen, Wen Hsiang
AU - Poongavanam, Mohan
AU - Dhamane, Sagar
AU - Stagg, Loren J.
AU - Ladbury, John E.
AU - Kourentzi, Katerina
AU - Strych, Ulrich
AU - Willson, Richard C.
PY - 2013/6
Y1 - 2013/6
N2 - The binding of the well-studied DNA aptamer aHt (5'-ATACCAGTCTATTCAATTGGGCCCGTCCGTAT GGTGGGTGTGCTGGCCAG-3'), which has been demonstrated to recognize human vascular endothelial growth factor (VEGF165) to recombinant VEGF was characterized using fluorescence anisotropy, isothermal titration calorimetry and analytical ultracentrifugation. The negatively-charged DNA aptamer is selective for VEGF and does not recognize positively-charged hen egg lysozyme, or bovine serum albumin. In contrast to the VEGF association of the previously-described aV DNA aptamer, where the binding is enthalpically driven and sequence-specific, the binding of the aHt aptamer to VEGF is entropically-driven and not abolished by scrambling of the sequence.
AB - The binding of the well-studied DNA aptamer aHt (5'-ATACCAGTCTATTCAATTGGGCCCGTCCGTAT GGTGGGTGTGCTGGCCAG-3'), which has been demonstrated to recognize human vascular endothelial growth factor (VEGF165) to recombinant VEGF was characterized using fluorescence anisotropy, isothermal titration calorimetry and analytical ultracentrifugation. The negatively-charged DNA aptamer is selective for VEGF and does not recognize positively-charged hen egg lysozyme, or bovine serum albumin. In contrast to the VEGF association of the previously-described aV DNA aptamer, where the binding is enthalpically driven and sequence-specific, the binding of the aHt aptamer to VEGF is entropically-driven and not abolished by scrambling of the sequence.
KW - Aptamer
KW - Fluorescence anisotropy
KW - Isothermal titration calorimetry
KW - Vascular endothelial growth factor
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U2 - 10.1016/j.ijbiomac.2013.02.016
DO - 10.1016/j.ijbiomac.2013.02.016
M3 - Article
C2 - 23470436
AN - SCOPUS:84875790899
VL - 57
SP - 69
EP - 75
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -