Biomimetic Molecular Signaling using DNA Walkers on Microparticles

Tulsi Ram Damase, Adam Spencer, Bamidele Samuel, Peter B Allen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or "track") for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.

Original languageEnglish (US)
Pages (from-to)4081
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Jun 22 2017

Keywords

  • Biomimetics/methods
  • DNA/chemistry
  • DNA, Catalytic
  • Fluorescent Dyes
  • Hydrogels/chemistry
  • Nanoparticles/chemistry
  • Nanotechnology

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