Biomimetic mineralization promotes viability and differentiation of human mesenchymal stem cells in a perfusion bioreactor

Gloria Belén Ramírez-Rodríguez, Ana Rita Pereira, Marietta Hermann, Jan Hansmann, José Manuel Delgado-López, Simone Sprio, Anna Tampieri, Monica Sandri

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the potential of the combination of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM.

Original languageEnglish (US)
Article number1447
Pages (from-to)1-15
Number of pages15
JournalInternational journal of molecular sciences
Issue number3
StatePublished - Feb 2 2021


  • Apatite nanoparticles
  • Collagen
  • Human mesenchymal stem cell
  • Magnesium
  • Osteogenesis
  • Perfusion bioreactor
  • Scaffold

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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