TY - JOUR
T1 - Biomimetic mineralization of recombinant collagen type I derived protein to obtain hybrid matrices for bone regeneration
AU - Ramírez-Rodríguez, Gloria Belén
AU - Delgado-López, José Manuel
AU - Iafisco, Michele
AU - Montesi, Monica
AU - Sandri, Monica
AU - Sprio, Simone
AU - Tampieri, Anna
N1 - Funding Information:
Authors would like to greatly acknowledge Dr. Silvia Panseri and Gopal Shankar for the support on the preliminary in vitro tests. We acknowledge Fujifilm Europe B.V. for the supply of Cellnest™. This study has been supported by EU Marie Curie Project “Bio-inspired Bone Regeneration” (BIO-INSPIRE: Grant agreement n°: 607051, FP7-PEOPLE-2013-ITN) and partially by the project BioBone (Andalucía Talent Hub, co-funded by Junta de Andalucía and FP7-Marie-Curie Actions).
Publisher Copyright:
© 2016 Elsevier Inc.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Understanding the mineralization mechanism of synthetic protein has recently aroused great interest especially in the development of advanced materials for bone regeneration. Herein, we propose the synthesis of composite materials through the mineralization of a recombinant collagen type I derived protein (RCP) enriched with RGD sequences in the presence of magnesium ions (Mg) to closer mimic bone composition. The role of both RCP and Mg ions in controlling the precipitation of the mineral phase is in depth evaluated. TEM and X-ray powder diffraction reveal the crystallization of nanocrystalline apatite (Ap) in all the evaluated conditions. However, Raman spectra point out also the precipitation of amorphous calcium phosphate (ACP). This amorphous phase is more evident when RCP and Mg are at work, indicating the synergistic role of both in stabilizing the amorphous precursor. In addition, hybrid matrices are prepared to tentatively address their effectiveness as scaffolds for bone tissue engineering. SEM and AFM imaging show an homogeneous mineral distribution on the RCP matrix mineralized in presence of Mg, which provides a surface roughness similar to that found in bone. Preliminary in vitro tests with pre-osteoblast cell line show good cell-material interaction on the matrices prepared in the presence of Mg. To the best of our knowledge this work represents the first attempt to mineralize recombinant collagen type I derived protein proving the simultaneous effect of the organic phase (RCP) and Mg on ACP stabilization. This study opens the possibility to engineer, through biomineralization process, advanced hybrid matrices for bone regeneration.
AB - Understanding the mineralization mechanism of synthetic protein has recently aroused great interest especially in the development of advanced materials for bone regeneration. Herein, we propose the synthesis of composite materials through the mineralization of a recombinant collagen type I derived protein (RCP) enriched with RGD sequences in the presence of magnesium ions (Mg) to closer mimic bone composition. The role of both RCP and Mg ions in controlling the precipitation of the mineral phase is in depth evaluated. TEM and X-ray powder diffraction reveal the crystallization of nanocrystalline apatite (Ap) in all the evaluated conditions. However, Raman spectra point out also the precipitation of amorphous calcium phosphate (ACP). This amorphous phase is more evident when RCP and Mg are at work, indicating the synergistic role of both in stabilizing the amorphous precursor. In addition, hybrid matrices are prepared to tentatively address their effectiveness as scaffolds for bone tissue engineering. SEM and AFM imaging show an homogeneous mineral distribution on the RCP matrix mineralized in presence of Mg, which provides a surface roughness similar to that found in bone. Preliminary in vitro tests with pre-osteoblast cell line show good cell-material interaction on the matrices prepared in the presence of Mg. To the best of our knowledge this work represents the first attempt to mineralize recombinant collagen type I derived protein proving the simultaneous effect of the organic phase (RCP) and Mg on ACP stabilization. This study opens the possibility to engineer, through biomineralization process, advanced hybrid matrices for bone regeneration.
KW - Biomineralization
KW - Bone
KW - Hydroxyapatite
KW - Magnesium
KW - Recombinant collagen derived protein
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U2 - 10.1016/j.jsb.2016.06.025
DO - 10.1016/j.jsb.2016.06.025
M3 - Article
C2 - 27374321
AN - SCOPUS:84996565850
VL - 196
SP - 138
EP - 146
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 2
ER -