TY - JOUR
T1 - Biomimetic Mg- and Mg,CO3-substituted hydroxyapatites
T2 - synthesis characterization and in vitro behaviour
AU - Landi, Elena
AU - Tampieri, Anna
AU - Mattioli-Belmonte, Monica
AU - Celotti, Giancarlo
AU - Sandri, Monica
AU - Gigante, Antonio
AU - Fava, Paola
AU - Biagini, Graziella
PY - 2006
Y1 - 2006
N2 - The doping of the apatite with carbonate or/and Mg ions in biologically-like amounts (6 and 1 wt.%, respectively) was performed. Chemico-physical characterizations and cell culture tests were carried out onto the synthetic Mg- and Mg,CO3-substituted (∼30-40 nm particle size) powders in comparison with stoichiometric HA (∼160 nm particle size) to determine as mesenchymal stem cells (MSCs) can directly use the mineral microenvironment to stimulate their own proliferation and differentiation activities. At the same time the growth of human osteoblast like cells (MG-63) was evaluated to determine the compatibility of the synthetic doped apatites for bone substitution. Cell morphology analysis by SEM as well as MTT and ALP tests were performed. The peculiar chemico-physical properties of the doped (Mg- and Mg,CO3-substituted) materials improved the behaviours of MSC and MG-63 cells in term of adhesion, proliferation and metabolic activation compared to stoichiometric HA.
AB - The doping of the apatite with carbonate or/and Mg ions in biologically-like amounts (6 and 1 wt.%, respectively) was performed. Chemico-physical characterizations and cell culture tests were carried out onto the synthetic Mg- and Mg,CO3-substituted (∼30-40 nm particle size) powders in comparison with stoichiometric HA (∼160 nm particle size) to determine as mesenchymal stem cells (MSCs) can directly use the mineral microenvironment to stimulate their own proliferation and differentiation activities. At the same time the growth of human osteoblast like cells (MG-63) was evaluated to determine the compatibility of the synthetic doped apatites for bone substitution. Cell morphology analysis by SEM as well as MTT and ALP tests were performed. The peculiar chemico-physical properties of the doped (Mg- and Mg,CO3-substituted) materials improved the behaviours of MSC and MG-63 cells in term of adhesion, proliferation and metabolic activation compared to stoichiometric HA.
KW - Apatite
KW - Biomedical applications
KW - Biomimetic materials
KW - Powders-chemical preparation
UR - http://www.scopus.com/inward/record.url?scp=33745617925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745617925&partnerID=8YFLogxK
U2 - 10.1016/j.jeurceramsoc.2005.06.040
DO - 10.1016/j.jeurceramsoc.2005.06.040
M3 - Article
AN - SCOPUS:33745617925
SN - 0955-2219
VL - 26
SP - 2593
EP - 2601
JO - Journal of the European Ceramic Society
JF - Journal of the European Ceramic Society
IS - 13
ER -