TY - JOUR
T1 - Biomimetic hydrogels for biosensor implant biocompatibility
T2 - Electrochemical characterization using micro-disc electrode arrays (MDEAs)
AU - Justin, Gusphyl
AU - Finley, Stephen
AU - Abdur Rahman, Abdur Rub
AU - Guiseppi-Elie, Anthony
N1 - Funding Information:
Funding This work was supported by the US Department of Defense (DoDPRMRP) grant PR023081/DAMD17-03-1-0172 and by the Consortium of the Clemson University Center for Bioelectronics, Biosensors and Biochips (C3B).
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009
Y1 - 2009
N2 - Our interest is in the development of engineered microdevices for continuous remote monitoring of intramuscular lactate, glucose, pH and temperature during post-traumatic hemorrhaging. Two important design considerations in the development of such devices for in vivo diagnostics are discussed; the utility of micro-disc electrode arrays (MDEAs) for electrochemical biosensing and the application of biomimetic, bioactive poly(HEMA)-based hydrogel composites for implant biocompatibility. A poly(HEMA)-based hydrogel membrane containing polyethylene glycol (PEG) was UV cross-linked with tetraethyleneglycol diacrylate following application to MDEAs (50 μm discs) and to 250 μm diameter gold electrodes within 8-well culture ware. Cyclic voltammetry (CV) of the MDEAs revealed a reduction in the apparent diffusion coefficient of ferrocenemonocarboxylic acid (FcCO2 H), from 6.68 × 10-5 to 6.74 × 10-6 cm2/s for the uncoated and 6 μm thick hydrogel coated devices, respectively. Single frequency (4 kHz) temporal impedance measurements of the hydrogels in the 8-well culture ware showed a reversible 5% change in the absolute impedance of the hydrogels when exposed to a pH change between 6.1 to 7.2 and a 20% drop between pH 6.1 and 8.8.
AB - Our interest is in the development of engineered microdevices for continuous remote monitoring of intramuscular lactate, glucose, pH and temperature during post-traumatic hemorrhaging. Two important design considerations in the development of such devices for in vivo diagnostics are discussed; the utility of micro-disc electrode arrays (MDEAs) for electrochemical biosensing and the application of biomimetic, bioactive poly(HEMA)-based hydrogel composites for implant biocompatibility. A poly(HEMA)-based hydrogel membrane containing polyethylene glycol (PEG) was UV cross-linked with tetraethyleneglycol diacrylate following application to MDEAs (50 μm discs) and to 250 μm diameter gold electrodes within 8-well culture ware. Cyclic voltammetry (CV) of the MDEAs revealed a reduction in the apparent diffusion coefficient of ferrocenemonocarboxylic acid (FcCO2 H), from 6.68 × 10-5 to 6.74 × 10-6 cm2/s for the uncoated and 6 μm thick hydrogel coated devices, respectively. Single frequency (4 kHz) temporal impedance measurements of the hydrogels in the 8-well culture ware showed a reversible 5% change in the absolute impedance of the hydrogels when exposed to a pH change between 6.1 to 7.2 and a 20% drop between pH 6.1 and 8.8.
KW - Arrays
KW - Biotransducers
KW - Electroanalysis
KW - Implantable biosensors
KW - Integrated circuits
UR - http://www.scopus.com/inward/record.url?scp=59949083629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59949083629&partnerID=8YFLogxK
U2 - 10.1007/s10544-008-9214-3
DO - 10.1007/s10544-008-9214-3
M3 - Article
C2 - 18679800
AN - SCOPUS:59949083629
VL - 11
SP - 103
EP - 115
JO - Biomedical Microdevices
JF - Biomedical Microdevices
SN - 1387-2176
IS - 1
ER -