Biomimetic hydrogels for biosensor implant biocompatibility: Electrochemical characterization using micro-disc electrode arrays (MDEAs)

Gusphyl Justin, Stephen Finley, Abdur Rub Abdur Rahman, Anthony Guiseppi-Elie

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Our interest is in the development of engineered microdevices for continuous remote monitoring of intramuscular lactate, glucose, pH and temperature during post-traumatic hemorrhaging. Two important design considerations in the development of such devices for in vivo diagnostics are discussed; the utility of micro-disc electrode arrays (MDEAs) for electrochemical biosensing and the application of biomimetic, bioactive poly(HEMA)-based hydrogel composites for implant biocompatibility. A poly(HEMA)-based hydrogel membrane containing polyethylene glycol (PEG) was UV cross-linked with tetraethyleneglycol diacrylate following application to MDEAs (50 μm discs) and to 250 μm diameter gold electrodes within 8-well culture ware. Cyclic voltammetry (CV) of the MDEAs revealed a reduction in the apparent diffusion coefficient of ferrocenemonocarboxylic acid (FcCO2 H), from 6.68 × 10-5 to 6.74 × 10-6 cm2/s for the uncoated and 6 μm thick hydrogel coated devices, respectively. Single frequency (4 kHz) temporal impedance measurements of the hydrogels in the 8-well culture ware showed a reversible 5% change in the absolute impedance of the hydrogels when exposed to a pH change between 6.1 to 7.2 and a 20% drop between pH 6.1 and 8.8.

Original languageEnglish (US)
Pages (from-to)103-115
Number of pages13
JournalBiomedical Microdevices
Volume11
Issue number1
DOIs
StatePublished - 2009

Keywords

  • Arrays
  • Biotransducers
  • Electroanalysis
  • Implantable biosensors
  • Integrated circuits

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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