Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs

Vittoria Di Mauro; Michele Iafisco; Nicolò Salvarani; Marco Vacchiano; Pierluigi Carullo; Gloria Belén Ramírez-Rodríguez; Tatiana Patrício; Anna Tampieri; Michele Miragoli; Daniele Catalucci

doi:10.2217/nnm.16.26

Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs

Vittoria Di Mauro, Michele Iafisco, Nicolò Salvarani, Marco Vacchiano, Pierluigi Carullo, Gloria Belén Ramírez-Rodríguez, Tatiana Patrício, Anna Tampieri, Michele Miragoli, Daniele Catalucci

Houston Methodist

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart. Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated. Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo. Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.

Original language	English (US)
Pages (from-to)	891-906
Number of pages	16
Journal	Nanomedicine
Volume	11
Issue number	8
DOIs	https://doi.org/10.2217/nnm.16.26
State	Published - Apr 2016

Keywords

calcium phosphate nanoparticles
cardiomyocytes
drug-delivery systems
microRNA
nanomedicine

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
General Materials Science

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title = "Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs",

abstract = "Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart. Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated. Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo. Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.",

keywords = "calcium phosphate nanoparticles, cardiomyocytes, drug-delivery systems, microRNA, nanomedicine",

author = "{Di Mauro}, Vittoria and Michele Iafisco and Nicol{\`o} Salvarani and Marco Vacchiano and Pierluigi Carullo and Ram{\'i}rez-Rodr{\'i}guez, {Gloria Bel{\'e}n} and Tatiana Patr{\'i}cio and Anna Tampieri and Michele Miragoli and Daniele Catalucci",

note = "Publisher Copyright: {\textcopyright} 2016 Future Medicine Ltd.",

year = "2016",

month = apr,

doi = "10.2217/nnm.16.26",

language = "English (US)",

volume = "11",

pages = "891--906",

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T1 - Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs

AU - Di Mauro, Vittoria

AU - Iafisco, Michele

AU - Salvarani, Nicolò

AU - Vacchiano, Marco

AU - Carullo, Pierluigi

AU - Ramírez-Rodríguez, Gloria Belén

AU - Patrício, Tatiana

AU - Tampieri, Anna

AU - Miragoli, Michele

AU - Catalucci, Daniele

PY - 2016/4

Y1 - 2016/4

N2 - Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart. Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated. Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo. Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.

AB - Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart. Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated. Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo. Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.

KW - calcium phosphate nanoparticles

KW - cardiomyocytes

KW - drug-delivery systems

KW - microRNA

KW - nanomedicine

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Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs

Abstract

Keywords

ASJC Scopus subject areas

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