Bioactivity and bone healing properties of biomimetic porous composite scaffold: In vitro and in vivo studies

Francesca Veronesi, Gianluca Giavaresi, Vincenzo Guarino, Maria Grazia Raucci, Monica Sandri, Anna Tampieri, Luigi Ambrosio, Milena Fini

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Tissue engineering (TE) represents a valid alternative to traditional surgical therapies for the management of bone defects that do not regenerate spontaneously. Scaffolds, one of the most important component of TE strategy, should be biocompatible, bioactive, osteoconductive, and osteoinductive. The aim of this study was to evaluate the biological properties and bone regeneration ability of a porous poly(ε-caprolactone) (PCL) scaffold, incorporating MgCO3-doped hydroxyapatite particles, uncoated (PCL-MgCHA) or coated by apatite-like crystals via biomimetic treatment (PCL-MgCHAB). It was observed that both scaffolds are not cytotoxic and, even if cell viability was similar on both scaffolds, PCL-MgCHAB showed higher alkaline phosphatase and collagen I (COLL I) production at day 7. PCL-MgCHA induced more tumor necrosis factor-α release than PCL-MgCHAB, while osteocalcin was produced less by both scaffolds up to 7 days and no significant differences were observed for transforming growth factor-β synthesis. The percentage of new bone trabeculae growth in wide defects carried out in rabbit femoral distal epiphyses was significantly higher in PCL-MgCHAB in comparison with PCL-MgCHA at 4 weeks and even more at 12 weeks after implantation. This study highlighted the role of a biomimetic composite scaffold in bone regeneration and lays the foundations for its future employment in the clinical practice.

Original languageEnglish (US)
Pages (from-to)2932-2941
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Issue number9
StatePublished - Sep 1 2015


  • biomaterials
  • bone cells
  • bone regeneration
  • composite scaffolds
  • histomorphometry

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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