Bioactive nanoengineered hydrogels for bone tissue engineering: A growth-factor-free approach

Janet R. Xavier, Teena Thakur, Prachi Desai, Manish K. Jaiswal, Nick Sears, Elizabeth Cosgriff-Hernandez, Roland Kaunas, Akhilesh K. Gaharwar

Research output: Contribution to journalArticle

340 Scopus citations

Abstract

Despite bones impressive ability to heal after traumatic injuries and fractures, a significant need still exists for developing strategies to promote healing of nonunion defects. To address this issue, we developed collagen-based hydrogels containing two-dimensional nanosilicates. Nanosilicates are ultrathin nanomaterials with a high degree of anisotropy and functionality that results in enhanced surface interactions with biological entities compared to their respective three-dimensional counterparts. The addition of nanosilicates resulted in a 4-fold increase in compressive modulus along with an increase in pore size compared to collagen-based hydrogels. In vitro evaluation indicated that the nanocomposite hydrogels are capable of promoting osteogenesis in the absence of any osteoinductive factors. A 3-fold increase in alkaline phosphatase activity and a 4-fold increase in the formation of a mineralized matrix were observed with the addition of the nanosilicates to the collagen-based hydrogels. Overall, these results demonstrate the multiple functions of nanosilicates conducive to the regeneration of bone in nonunion defects, including increased network stiffness and porosity, injectability, and enhanced mineralized matrix formation in a growth-factor-free microenvironment.

Original languageEnglish (US)
Pages (from-to)3109-3118
Number of pages10
JournalACS Nano
Volume9
Issue number3
DOIs
StatePublished - Mar 24 2015

Keywords

  • bone regeneration
  • nanocomposite hydrogels
  • scaffolds
  • synthetic nanosilicates
  • tissue engineering
  • two-dimensional (2D) nanoparticles

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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