Bioactive electroconductive hydrogels yield novel biotransducers for glucose

Christian N. Kotanen, Anthony Guiseppi-Elie

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A class of bioactive, stimuli-responsive co-joined interpenetrating networks of inherently conductive polymers and highly hydrated hydrogels are being developed for implantable biodevice interfaces and for electric field induced release of elutable drugs. A novel microfabricated multidisc electrode array biotransducer intended for trauma management has been coated with poly(HEMA)-polypyrrole and characterized by cyclic voltammetry and chronoamperometry using ferrocene monocarboxylic acid (FcCOOH) as a probe molecule. Electrodeposition of polypyrrole (700 mV vs. Ag/AgCl) to 100 mC/cm 2 onto the hydrogel coated microdisc electrode array resulted in large and unstable background currents relative to uncoated electrodes. Overoxidation of polypyrrole (0-1.2 V vs. Ag/AgCl, 20 cylces, 100 mV/s) eliminates background current. Dose-response curves with FcCOOH showed that the transducer has good reproducibility with molecules of facile electrochemical properties. Polypyrrole provides interference screening of endogenous interferents in biosensor applications with a 12:1 rejection ratio. GOx was immobilized via electropolymerization of polypyrrole into hydrogel coated MDEA 5037s to yield biotransducers with sensitivity of 0.045 μA mM -1 cm -2. Changes to improve biotransducer sensitivity are proposed.

Original languageEnglish (US)
Pages (from-to)187-197
Number of pages11
JournalMacromolecular Symposia
Volume317-318
Issue number1
DOIs
StatePublished - Aug 2012

Keywords

  • biosensors
  • conductive polymers
  • electrodes
  • enzymes
  • hydrogels
  • microarrays

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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