Abstract
A class of bioactive, stimuli-responsive co-joined interpenetrating networks of inherently conductive polymers and highly hydrated hydrogels are being developed for implantable biodevice interfaces and for electric field induced release of elutable drugs. A novel microfabricated multidisc electrode array biotransducer intended for trauma management has been coated with poly(HEMA)-polypyrrole and characterized by cyclic voltammetry and chronoamperometry using ferrocene monocarboxylic acid (FcCOOH) as a probe molecule. Electrodeposition of polypyrrole (700 mV vs. Ag/AgCl) to 100 mC/cm 2 onto the hydrogel coated microdisc electrode array resulted in large and unstable background currents relative to uncoated electrodes. Overoxidation of polypyrrole (0-1.2 V vs. Ag/AgCl, 20 cylces, 100 mV/s) eliminates background current. Dose-response curves with FcCOOH showed that the transducer has good reproducibility with molecules of facile electrochemical properties. Polypyrrole provides interference screening of endogenous interferents in biosensor applications with a 12:1 rejection ratio. GOx was immobilized via electropolymerization of polypyrrole into hydrogel coated MDEA 5037s to yield biotransducers with sensitivity of 0.045 μA mM -1 cm -2. Changes to improve biotransducer sensitivity are proposed.
Original language | English (US) |
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Pages (from-to) | 187-197 |
Number of pages | 11 |
Journal | Macromolecular Symposia |
Volume | 317-318 |
Issue number | 1 |
DOIs | |
State | Published - Aug 2012 |
Keywords
- biosensors
- conductive polymers
- electrodes
- enzymes
- hydrogels
- microarrays
ASJC Scopus subject areas
- Condensed Matter Physics
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry