TY - JOUR
T1 - Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2
AU - Fernandes Patrício, Tatiana M.
AU - Mumcuoglu, Didem
AU - Montesi, Monica
AU - Panseri, Silvia
AU - Witte-Bouma, Janneke
AU - Garcia, Shorouk Fahmy
AU - Sandri, Monica
AU - Tampieri, Anna
AU - Farrell, Eric
AU - Sprio, Simone
N1 - Funding Information:
The research leading to these results has received funding from the European Union Seventh Framework Programme FP7-PEOPLE-2013-ITN under grant agreement n° 607051 .
Funding Information:
The authors acknowledge to Professor Gerjo van Osch from Department of Orthopaedics, Erasmus MC (Rotterdam, the Netherlands) for the support and research advice, during the secondment in Erasmus MC; to Fujifilm Manufacturing Europe B.V. (The Netherlands) for providing the Cellnest™; to Joachim Nickel from Department for Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Germany and Fraunhofer IGB, Translational Center Würzburg, Germany, for providing the rhBMP-2; to Andreana Piancastelli and Giulio Boveri from Institute of Science and Technology for Ceramics (ISTEC-CNR), for porosity and contact angle measurements, respectively. The research leading to these results has received funding from the European Union Seventh Framework Programme FP7-PEOPLE-2013-ITN under grant agreement n° 607051.
Funding Information:
Didem Mumcuoglu started her PhD in Netherlands in 2014. Her PhD study was funded by Marie Curie ITN (Bio-inspire) involving Fujifilm and Erasmus Medical Center as project partners. Her role in the consortium was to develop a growth factor delivery system for bone regeneration under supervision of Dr. Sebastiaan Kluijtmans and her PhD promoter Prof. Gerjo van Osch. After finishing her PhD she started as a post-doc in Prof. Patricia Danker's group. Her current research is focused on designing a device based on supramolecular polymers for islet cell transplantation.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2
Y1 - 2021/2
N2 - Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release profiles. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic microspheres in precisely designed and personalized therapies.
AB - Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release profiles. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic microspheres in precisely designed and personalized therapies.
KW - Bone regeneration
KW - Hybrid superparamagnetic microspheres
KW - Osteogenesis
KW - Pulsed electromagnetic field
KW - Sustained release
KW - rhBMP-2
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U2 - 10.1016/j.msec.2020.111410
DO - 10.1016/j.msec.2020.111410
M3 - Article
C2 - 33321577
AN - SCOPUS:85090279993
VL - 119
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
M1 - 111410
ER -