Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2

Tatiana M. Fernandes Patrício, Didem Mumcuoglu, Monica Montesi, Silvia Panseri, Janneke Witte-Bouma, Shorouk Fahmy Garcia, Monica Sandri, Anna Tampieri, Eric Farrell, Simone Sprio

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release profiles. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic microspheres in precisely designed and personalized therapies.

Original languageEnglish (US)
Article number111410
JournalMaterials Science and Engineering C
StatePublished - Feb 2021


  • Bone regeneration
  • Hybrid superparamagnetic microspheres
  • Osteogenesis
  • Pulsed electromagnetic field
  • Sustained release
  • rhBMP-2

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials


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