TY - JOUR
T1 - Binding of heparin and heparan sulphate to rat liver cells
AU - Kjellén, Lena
AU - Oldberg, Åke
AU - Rubin, Kristofer
AU - Hook, Magnus
PY - 1977/1/10
Y1 - 1977/1/10
N2 - The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis. The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction. The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells.
AB - The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis. The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction. The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells.
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U2 - 10.1016/0006-291X(77)91384-5
DO - 10.1016/0006-291X(77)91384-5
M3 - Article
C2 - 138421
AN - SCOPUS:0017328431
VL - 74
SP - 126
EP - 133
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -