Binding of heparin and heparan sulphate to rat liver cells

Lena Kjellén; Åke Oldberg; Kristofer Rubin; Magnus Hook

doi:10.1016/0006-291X(77)91384-5

Binding of heparin and heparan sulphate to rat liver cells

Lena Kjellén, Åke Oldberg, Kristofer Rubin, Magnus Hook

Research Institute

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77 Scopus citations

Abstract

The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis. The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction. The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells.

Original language	English (US)
Pages (from-to)	126-133
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	74
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(77)91384-5
State	Published - Jan 10 1977

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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T1 - Binding of heparin and heparan sulphate to rat liver cells

AU - Kjellén, Lena

AU - Oldberg, Åke

AU - Rubin, Kristofer

AU - Hook, Magnus

PY - 1977/1/10

Y1 - 1977/1/10

N2 - The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis. The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction. The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells.

AB - The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis. The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction. The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells.

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