BHLHB4 is a bHLH transcriptional regulator in pancreas and brain that marks the dimesencephalic boundary

Debra E. Bramblett; Neal G. Copeland; Nancy A. Jenkins; Ming Jer Tsai

doi:10.1006/geno.2002.6708

BHLHB4 is a bHLH transcriptional regulator in pancreas and brain that marks the dimesencephalic boundary

Debra E. Bramblett, Neal G. Copeland, Nancy A. Jenkins, Ming Jer Tsai

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35 Scopus citations

Abstract

We have cloned a basic helix-loop-helix (bHLH) factor gene, Bh1hb4, from a mouse β-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bh1hb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new subgroup of bHLH factors including at least four previously identified mouse bHLH factors: BHLHB5, MIST1, OLIG1, OLIG2, and OLIG3. In the developing nervous system, Bh1hb4 was found to mark the dimesencephalic boundary, suggesting that Bh1hb4 may have a role in diencephalic regionalization. In the pancreas, Bh1hb4 is expressed in a transient fashion that suggests a role in the pancreatic endocrine cell lineage. Transfection experiments show that BHLHB4 can repress transcriptional activation mediated through the pancreatic β-cell specific insulin promoter enhancer RIPE3. Together, these data suggest that BHLHB4 may modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types.

Original language	English (US)
Pages (from-to)	402-412
Number of pages	11
Journal	Genomics
Volume	79
Issue number	3
DOIs	https://doi.org/10.1006/geno.2002.6708
State	Published - 2002

Keywords

Basic helix-loop-helix protein
Development
Diencephalon
Pancreatic β-cells
Phylogeny
Physical chromosome mapping
Transcription factor

ASJC Scopus subject areas

Genetics

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10.1006/geno.2002.6708

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@article{cf463d3254224a8b9fa054faa88182c1,

title = "BHLHB4 is a bHLH transcriptional regulator in pancreas and brain that marks the dimesencephalic boundary",

abstract = "We have cloned a basic helix-loop-helix (bHLH) factor gene, Bh1hb4, from a mouse β-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bh1hb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new subgroup of bHLH factors including at least four previously identified mouse bHLH factors: BHLHB5, MIST1, OLIG1, OLIG2, and OLIG3. In the developing nervous system, Bh1hb4 was found to mark the dimesencephalic boundary, suggesting that Bh1hb4 may have a role in diencephalic regionalization. In the pancreas, Bh1hb4 is expressed in a transient fashion that suggests a role in the pancreatic endocrine cell lineage. Transfection experiments show that BHLHB4 can repress transcriptional activation mediated through the pancreatic β-cell specific insulin promoter enhancer RIPE3. Together, these data suggest that BHLHB4 may modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types.",

keywords = "Basic helix-loop-helix protein, Development, Diencephalon, Pancreatic β-cells, Phylogeny, Physical chromosome mapping, Transcription factor",

author = "Bramblett, {Debra E.} and Copeland, {Neal G.} and Jenkins, {Nancy A.} and Tsai, {Ming Jer}",

note = "Funding Information: We thank Antonio Baldini and Vesna Jurecic, at Baylor College of Medicine, for assistance with FISH analysis; Deborah B. Householder (National Cancer Institute at Frederick) for technical assistance associated with the genetic mapping; and Khoi Chu, Fred A. Pereira, Fabrice Petit, Sophia Y. Tsai, and Cheng Zhou (all from Baylor College of Medicine) for helpful scientific discussion and/or critical reading of the manuscript. This work was supported by a fellowship from the Juvenile Diabetes Foundation International and grant from NIDDK(D.E.B.) and grants from NIH (M.-J.T.). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2002",

doi = "10.1006/geno.2002.6708",

language = "English (US)",

volume = "79",

pages = "402--412",

journal = "Genomics",

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publisher = "Academic Press",

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T1 - BHLHB4 is a bHLH transcriptional regulator in pancreas and brain that marks the dimesencephalic boundary

AU - Bramblett, Debra E.

AU - Copeland, Neal G.

AU - Jenkins, Nancy A.

AU - Tsai, Ming Jer

N1 - Funding Information: We thank Antonio Baldini and Vesna Jurecic, at Baylor College of Medicine, for assistance with FISH analysis; Deborah B. Householder (National Cancer Institute at Frederick) for technical assistance associated with the genetic mapping; and Khoi Chu, Fred A. Pereira, Fabrice Petit, Sophia Y. Tsai, and Cheng Zhou (all from Baylor College of Medicine) for helpful scientific discussion and/or critical reading of the manuscript. This work was supported by a fellowship from the Juvenile Diabetes Foundation International and grant from NIDDK(D.E.B.) and grants from NIH (M.-J.T.). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - We have cloned a basic helix-loop-helix (bHLH) factor gene, Bh1hb4, from a mouse β-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bh1hb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new subgroup of bHLH factors including at least four previously identified mouse bHLH factors: BHLHB5, MIST1, OLIG1, OLIG2, and OLIG3. In the developing nervous system, Bh1hb4 was found to mark the dimesencephalic boundary, suggesting that Bh1hb4 may have a role in diencephalic regionalization. In the pancreas, Bh1hb4 is expressed in a transient fashion that suggests a role in the pancreatic endocrine cell lineage. Transfection experiments show that BHLHB4 can repress transcriptional activation mediated through the pancreatic β-cell specific insulin promoter enhancer RIPE3. Together, these data suggest that BHLHB4 may modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types.

AB - We have cloned a basic helix-loop-helix (bHLH) factor gene, Bh1hb4, from a mouse β-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bh1hb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new subgroup of bHLH factors including at least four previously identified mouse bHLH factors: BHLHB5, MIST1, OLIG1, OLIG2, and OLIG3. In the developing nervous system, Bh1hb4 was found to mark the dimesencephalic boundary, suggesting that Bh1hb4 may have a role in diencephalic regionalization. In the pancreas, Bh1hb4 is expressed in a transient fashion that suggests a role in the pancreatic endocrine cell lineage. Transfection experiments show that BHLHB4 can repress transcriptional activation mediated through the pancreatic β-cell specific insulin promoter enhancer RIPE3. Together, these data suggest that BHLHB4 may modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types.

KW - Basic helix-loop-helix protein

KW - Development

KW - Diencephalon

KW - Pancreatic β-cells

KW - Phylogeny

KW - Physical chromosome mapping

KW - Transcription factor

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DO - 10.1006/geno.2002.6708

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C2 - 11863370

AN - SCOPUS:0036200920

SN - 0888-7543

VL - 79

SP - 402

EP - 412

JO - Genomics

JF - Genomics

IS - 3

ER -

BHLHB4 is a bHLH transcriptional regulator in pancreas and brain that marks the dimesencephalic boundary

Abstract

Keywords

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