Betulinic acid targets YY1 and ErbB2 through cannabinoid receptor-dependent disruption of microRNA-27a:ZBTB10 in breast cancer

Xinyi Liu, Indira Jutooru, Ping Lei, Kyoung Hyun Kim, Syng Ook Lee, Lisa K. Brents, Paul L. Prather, Stephen Safe

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Treatment of ErbB2-overexpressing BT474 and MDA-MB-453 breast cancer cells with 1 to 10 mmol/L betulinic acid inhibited cell growth, induced apoptosis, downregulated specificity protein (Sp) transcription factors Sp1, Sp3, and Sp4, and decreased expression of ErbB2. Individual or combined knockdown of Sp1, Sp3, Sp4 by RNA interference also decreased expression of ErbB2 and this response was because of repression of YY1, an Sp-regulated gene. Betulinic acid-dependent repression of Sp1, Sp3, Sp4, and Sp-regulated genes was due, in part, to induction of the Sp repressor ZBTB10 and downregulation of microRNA-27a (miR-27a), which constitutively inhibits ZBTB10 expression, and we show for the first time that the effects of betulinic acid on the miR-27a:ZBTB10-Sp transcription factor axis were cannabinoid 1 (CB1) and CB2 receptor-dependent, thus identifying a new cellular target for this anticancer agent.

Original languageEnglish (US)
Pages (from-to)1421-1431
Number of pages11
JournalMolecular Cancer Therapeutics
Volume11
Issue number7
DOIs
StatePublished - Jul 2012

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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