Behavior of collagen-GAG matrices as dermal replacement in rodent and porcine models

Dennis P. Orgill, Charles E. Butler, John F. Regan

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The behavior of a highly specific and active collagen-glycosaminoglycan (CG) copolymer was evaluated in full-thickness wounds of Hartley guinea pigs and Yorkshire pigs. Several CG copolymers of different physiochemical properties have been previously applied in vivo in guinea pigs. The skin of the Yorkshire pig is more similar to human skin and allows comparision of multiple wound sites on the same animal. Gross and histologic observations of CG treated wounds were made over a three week period. The results showed significant differences in wound contraction, cellular infiltration, and matrix degradation between the two models. Wound contraction, measured as a percent of original wound area, was delayed by the CG matrix in both models compared to full-thickness control wounds. At 21 days, guinea pig wounds treated with the CG matrix had contracted to 49 ± 14 percent of the original area compared with 13 ± 6 percent in ungrafted controls. At the same time point in porcine wounds, much less wound contraction was observed, 91 ± 7 percent of the original area in CG grafted wounds versus 76 ± 7 percent in open wounds. CG matrices seem to alter the kinetics of wound closure in both the guinea pig model, which heals full-thickness dermal wounds primarily by contraction, and the porcine model, which heals primarily by epithelialization. Histologic observations showed both cellular infiltration and matrix degradation to occur more rapidly in the porcine model. Both models provide important in vivo information on the biologic response of these matrices.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalWounds
Volume8
Issue number5
StatePublished - Sep 1 1996

ASJC Scopus subject areas

  • Surgery
  • Medical–Surgical

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