Bcl-x(L) overexpression inhibits progression of molecular events leading to paclitaxel-induced apoptosis of human acute myeloid leukemia HL-60 cells

Ana Maria Ibrado, Linda Liu, Kapil Bhalla

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Paclitaxel has been shown to activate Raf-1 and cause phosphorylation of Bcl-2, which has been correlated with paclitaxel-induced apoptosis of cancer cells. In the present studies, we demonstrate that in human AML HL-60 cells that express Bcl-2 but little Bcl-x(L) (HL-60/neo cells), paclitaxel-induced phosphorylation of Bcl-2 is followed by increased intracellular free Bax levels. This, in turn, is followed by the cleavage and activation of the key cysteine protease, CPP32β/Yama, and cleavage of poly(ADP-ribose) polymerase, resulting in the DNA fragmentation of apoptosis. Cotreatment with the benzoquinone ansamycin Geldanamycin depleted Raf-1 but did not decrease Bcl- 2 levels or impair paclitaxel-induced Bcl-2 phosphorylation in HL-60/neo cells. Also, Geldanamycin did not affect paclitaxel-induced apoptosis of HL- 60/neo cells. As compared to the control HL-60/neo, HL-60/Bcl-X(L) cells contain Bcl-2 as well as an enforced overexpression of Bcl-x(L). Immunoprecipitation studies with anti-Bcl-2 and/or anti-Bcl-x antibodies demonstrated that HL-60/Bcl-x(L) cells possess lower free Bax but higher levels of Bax heterodimerized to Bcl-2 and Bcl-x(L). Following treatment of HL-60/Bcl-x(L) cells with paclitaxel, although Bcl-2 phosphorylation was observed, it was not followed by increased free Bax levels, cleavage of CPP32β/Yama and poly(ADP-ribose) polymerase, or induction of the DNA fragmentation of apoptosis. These findings indicate the order of molecular events leading to paclitaxel-induced apoptosis and show that Raf-1 may not be involved in paclitaxel-induced phosphorylation of Bcl-2 or apoptosis of HL- 60 cells.

Original languageEnglish (US)
Pages (from-to)1109-1115
Number of pages7
JournalCancer research
Volume57
Issue number6
StatePublished - Apr 9 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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