Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat: Immunocytochemical analysis in combination with stereology

A. Cintra; J. Lindberg; G. Chadi; B. Tinner; A. Möller; J. Å Gustafsson; E. R. DeKloet; M. Oitzl; K. Nishikawa; L. F. Agnati; K. Fuxe

doi:10.1016/0197-0186(94)90051-5

Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat: Immunocytochemical analysis in combination with stereology

A. Cintra, J. Lindberg, G. Chadi, B. Tinner, A. Möller, J. Å Gustafsson, E. R. DeKloet, M. Oitzl, K. Nishikawa, L. F. Agnati, K. Fuxe

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Abstract

The effect of aging on the hippocampal formation of the male Brown Norway rat was studied by immunohistochemistry and measurements of the immunoreactive hippocampal cells using stereological techniques. The total estimated number of glucocorticoid receptor (GR) immunoreactive neurons of the CA1-CA2 area did not differ in the 3- and the 36-month-old rat. However, the intensity of the GR immunoreactivity was decreased in the aged animals. A gradual decrease of the immunoreactivity for the mineralocorticoid receptor was also observed in the CA1-CA2 area. In the stratum oriens and the stratum radiatum of the CA1-CA2 area the immunoreactivity for basic fibroblast growth factor (bFGF) present in the glia was found to be reduced [20,000 ± 2100 (n = 6)] in the 36-month-old rat vs the 3-month-old rat [28,500 ± 4500 (n = 4) (^*P = 0.05)]. However, there was no difference in the number of glial fibrillary acidic protein immunoreactive cells of this area in these two age groups. The present findings give evidence that in the Brown Norway rat there is no loss of the neuronal population containing glucocorticoid receptors of the CA1-CA2 area during aging but suggest that aging is characterized by deficits of glially derived growth factors, such as bFGF.

Original language	English (US)
Pages (from-to)	39-45
Number of pages	7
Journal	Neurochemistry International
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0197-0186(94)90051-5
State	Published - Jul 1994

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Cell Biology

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10.1016/0197-0186(94)90051-5

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Cintra, A., Lindberg, J., Chadi, G., Tinner, B., Möller, A., Gustafsson, J. Å., DeKloet, E. R., Oitzl, M., Nishikawa, K., Agnati, L. F., & Fuxe, K. (1994). Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat: Immunocytochemical analysis in combination with stereology. Neurochemistry International, 25(1), 39-45. https://doi.org/10.1016/0197-0186(94)90051-5

Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat : Immunocytochemical analysis in combination with stereology. / Cintra, A.; Lindberg, J.; Chadi, G.; Tinner, B.; Möller, A.; Gustafsson, J. Å; DeKloet, E. R.; Oitzl, M.; Nishikawa, K.; Agnati, L. F.; Fuxe, K.

In: Neurochemistry International, Vol. 25, No. 1, 07.1994, p. 39-45.

Research output: Contribution to journal › Article › peer-review

Cintra, A, Lindberg, J, Chadi, G, Tinner, B, Möller, A, Gustafsson, JÅ, DeKloet, ER, Oitzl, M, Nishikawa, K, Agnati, LF & Fuxe, K 1994, 'Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat: Immunocytochemical analysis in combination with stereology', Neurochemistry International, vol. 25, no. 1, pp. 39-45. https://doi.org/10.1016/0197-0186(94)90051-5

Cintra, A. ; Lindberg, J. ; Chadi, G. ; Tinner, B. ; Möller, A. ; Gustafsson, J. Å ; DeKloet, E. R. ; Oitzl, M. ; Nishikawa, K. ; Agnati, L. F. ; Fuxe, K. / Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat : Immunocytochemical analysis in combination with stereology. In: Neurochemistry International. 1994 ; Vol. 25, No. 1. pp. 39-45.

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title = "Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat: Immunocytochemical analysis in combination with stereology",

abstract = "The effect of aging on the hippocampal formation of the male Brown Norway rat was studied by immunohistochemistry and measurements of the immunoreactive hippocampal cells using stereological techniques. The total estimated number of glucocorticoid receptor (GR) immunoreactive neurons of the CA1-CA2 area did not differ in the 3- and the 36-month-old rat. However, the intensity of the GR immunoreactivity was decreased in the aged animals. A gradual decrease of the immunoreactivity for the mineralocorticoid receptor was also observed in the CA1-CA2 area. In the stratum oriens and the stratum radiatum of the CA1-CA2 area the immunoreactivity for basic fibroblast growth factor (bFGF) present in the glia was found to be reduced [20,000 ± 2100 (n = 6)] in the 36-month-old rat vs the 3-month-old rat [28,500 ± 4500 (n = 4) (*P = 0.05)]. However, there was no difference in the number of glial fibrillary acidic protein immunoreactive cells of this area in these two age groups. The present findings give evidence that in the Brown Norway rat there is no loss of the neuronal population containing glucocorticoid receptors of the CA1-CA2 area during aging but suggest that aging is characterized by deficits of glially derived growth factors, such as bFGF.",

author = "A. Cintra and J. Lindberg and G. Chadi and B. Tinner and A. M{\"o}ller and Gustafsson, {J. {\AA}} and DeKloet, {E. R.} and M. Oitzl and K. Nishikawa and Agnati, {L. F.} and K. Fuxe",

note = "Funding Information: Acknowledgements--The authors are grateful to Dr Z. Kro-zowski for supplying the MR antiserum and to Professor K. Nishikawa for providing the monoclonal antibody against bFGF. This work has been supported by grants (04X-715) from the Swedish Medical Research Council, from L. Oster-man's Foundation, from Lars Hierta's Minne Foundation (A.C.), from the Swedish Medical Society (A.C.) and FAPESP 91/2989-9, Sao Paulo, Brazil (G.C.). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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doi = "10.1016/0197-0186(94)90051-5",

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T1 - Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat

T2 - Immunocytochemical analysis in combination with stereology

AU - Cintra, A.

AU - Lindberg, J.

AU - Chadi, G.

AU - Tinner, B.

AU - Möller, A.

AU - Gustafsson, J. Å

AU - DeKloet, E. R.

AU - Oitzl, M.

AU - Nishikawa, K.

AU - Agnati, L. F.

AU - Fuxe, K.

N1 - Funding Information: Acknowledgements--The authors are grateful to Dr Z. Kro-zowski for supplying the MR antiserum and to Professor K. Nishikawa for providing the monoclonal antibody against bFGF. This work has been supported by grants (04X-715) from the Swedish Medical Research Council, from L. Oster-man's Foundation, from Lars Hierta's Minne Foundation (A.C.), from the Swedish Medical Society (A.C.) and FAPESP 91/2989-9, Sao Paulo, Brazil (G.C.). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1994/7

Y1 - 1994/7

N2 - The effect of aging on the hippocampal formation of the male Brown Norway rat was studied by immunohistochemistry and measurements of the immunoreactive hippocampal cells using stereological techniques. The total estimated number of glucocorticoid receptor (GR) immunoreactive neurons of the CA1-CA2 area did not differ in the 3- and the 36-month-old rat. However, the intensity of the GR immunoreactivity was decreased in the aged animals. A gradual decrease of the immunoreactivity for the mineralocorticoid receptor was also observed in the CA1-CA2 area. In the stratum oriens and the stratum radiatum of the CA1-CA2 area the immunoreactivity for basic fibroblast growth factor (bFGF) present in the glia was found to be reduced [20,000 ± 2100 (n = 6)] in the 36-month-old rat vs the 3-month-old rat [28,500 ± 4500 (n = 4) (*P = 0.05)]. However, there was no difference in the number of glial fibrillary acidic protein immunoreactive cells of this area in these two age groups. The present findings give evidence that in the Brown Norway rat there is no loss of the neuronal population containing glucocorticoid receptors of the CA1-CA2 area during aging but suggest that aging is characterized by deficits of glially derived growth factors, such as bFGF.

AB - The effect of aging on the hippocampal formation of the male Brown Norway rat was studied by immunohistochemistry and measurements of the immunoreactive hippocampal cells using stereological techniques. The total estimated number of glucocorticoid receptor (GR) immunoreactive neurons of the CA1-CA2 area did not differ in the 3- and the 36-month-old rat. However, the intensity of the GR immunoreactivity was decreased in the aged animals. A gradual decrease of the immunoreactivity for the mineralocorticoid receptor was also observed in the CA1-CA2 area. In the stratum oriens and the stratum radiatum of the CA1-CA2 area the immunoreactivity for basic fibroblast growth factor (bFGF) present in the glia was found to be reduced [20,000 ± 2100 (n = 6)] in the 36-month-old rat vs the 3-month-old rat [28,500 ± 4500 (n = 4) (*P = 0.05)]. However, there was no difference in the number of glial fibrillary acidic protein immunoreactive cells of this area in these two age groups. The present findings give evidence that in the Brown Norway rat there is no loss of the neuronal population containing glucocorticoid receptors of the CA1-CA2 area during aging but suggest that aging is characterized by deficits of glially derived growth factors, such as bFGF.

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Basic fibroblast growth factor and steroid receptors in the aging hippocampus of the Brown Norway rat: Immunocytochemical analysis in combination with stereology

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