Base Pairing and Template Specificity During Deoxyribonucleic Acid Repair Synthesis in Human and Mouse Cells

Michael W. Lieberman, Miriam C. Poirier

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Nuclcotide insertion during DNA repair synthesis has been studied in cultured human diploid fibroblasts (WI-38) and Balb/c 3T3 mouse cells damaged with ultraviolet radiation and two mutagenic carcinogens, N-acetoxy-2-acetylaminofluorene (NA-AAF) and 7-bromomethylbenz[a]anthracene (7BrMeBA). Evidence from thermal elution chromatography, S1 nuclease digestion studies, and pyrimidine isostich analysis suggests that in mammalian cells the complementary, undamaged strand serves as a template for repair synthesis and that the result of such synthesis is the accurate restoration of the damaged area to the original nucleotide sequence.

Original languageEnglish (US)
Pages (from-to)5384-5388
Number of pages5
JournalBiochemistry
Volume13
Issue number26
DOIs
StatePublished - Dec 1 1974

ASJC Scopus subject areas

  • Biochemistry

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