@article{bd8096dfaa064c54bc63b2bcccee5a4b,
title = "Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness",
abstract = "Bioengineering advances have made it possible to fundamentally alter the genetic codes of organisms. However, the evolutionary consequences of expanding an organism's genetic code with a noncanonical amino acid are poorly understood. Here we show that bacteriophages evolved on a host that incorporates 3-iodotyrosine at the amber stop codon acquire neutral and beneficial mutations to this new amino acid in their proteins, demonstrating that an expanded genetic code increases evolvability.",
author = "Hammerling, {Michael J.} and Ellefson, {Jared W.} and Boutz, {Daniel R.} and Marcotte, {Edward M.} and Ellington, {Andrew D.} and Barrick, {Jeffrey E.}",
note = "Funding Information: We thank J. Bull, I. Molineux, R. Hughes, C. Barnhart, D. Deatherage, R. Alnahhas, M. Schmerer, A. Miklos, A. Meyer and A. Maranh{\~a}o (all at the University of Texas at Austin) for plasmids, strains, advice and technical assistance. The RF0 IodoY strain was provided by RIKEN and by the Targeted Proteins Research Program, the Ministry of Education, Culture, Sports, Science and Technology, Japan. We acknowledge the Texas Advanced Computing Center (TACC) at The University of Texas at Austin for providing HPC resources. This research was supported by the US National Institutes of Health (NIH) (R00-GM087550 to J.E.B.), the US National Science Foundation (NSF) BEACON Center for the Study of Evolution in Action (DBI-0939454 to J.E.B.), the US Army Research Office (W911NF-12-1-0390 to J.E.B. and E.M.M.), the US National Security Science and Engineering Faculty (FA9550-10-1-01-69 to A.D.E.), the US Defense Advanced Research Project Agency (HR-0011-12-C-0066 to A.D.E.) and the NSF (MCB-0943383 to A.D.E.). E.M.M. also acknowledges funding from the NIH, the NSF, the Cancer Prevention Research Institute of Texas and the Welch Foundation (F1515).",
year = "2014",
month = mar,
doi = "10.1038/nchembio.1450",
language = "English (US)",
volume = "10",
pages = "178--180",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "3",
}