Bacteriophage-antibiotic combinations for enterococcus faecium with varying bacteriophage and daptomycin susceptibilities

Taylor Morrisette; Katherine L. Lev; Razieh Kebriaei; Jacinda C. Abdul-Mutakabbir; Kyle C. Stamper; Sandra Morales; Susan M. Lehman; Gregory S. Canfield; Breck A. Duerkop; Cesar A. Arias; Michael J. Rybak

doi:10.1128/AAC.00993-20

Bacteriophage-antibiotic combinations for enterococcus faecium with varying bacteriophage and daptomycin susceptibilities

Taylor Morrisette, Katherine L. Lev, Razieh Kebriaei, Jacinda C. Abdul-Mutakabbir, Kyle C. Stamper, Sandra Morales, Susan M. Lehman, Gregory S. Canfield, Breck A. Duerkop, Cesar A. Arias, Michael J. Rybak

Houston Methodist

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Concerns regarding increased prevalence of daptomycin (DAP)-resistant strains necessitate novel therapies for Enterococcus faecium infections. Obligately lytic bacteriophages are viruses that target, infect, and kill bacterial cells. Limited studies have evaluated phage-antibiotic combinations against E. faecium. After an initial screen of eight E. faecium strains, three strains with varying DAP/phage susceptibilities were selected for further experiments. Phage-to-strain specificity contributed to synergy with antibiotics by time-kill analyses and was associated with lower development of phage resistance.

Original language	English (US)
Article number	e00993-20
Journal	Antimicrobial Agents and Chemotherapy
Volume	64
Issue number	9
DOIs	https://doi.org/10.1128/AAC.00993-20
State	Published - Sep 2020

Keywords

Bacteriophage-antibiotic combinations
Bacteriophages
Daptomycin
Enterococcus faecium

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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Morrisette, T., Lev, K. L., Kebriaei, R., Abdul-Mutakabbir, J. C., Stamper, K. C., Morales, S., Lehman, S. M., Canfield, G. S., Duerkop, B. A., Arias, C. A., & Rybak, M. J. (2020). Bacteriophage-antibiotic combinations for enterococcus faecium with varying bacteriophage and daptomycin susceptibilities. Antimicrobial Agents and Chemotherapy, 64(9), Article e00993-20. https://doi.org/10.1128/AAC.00993-20

Morrisette, T, Lev, KL, Kebriaei, R, Abdul-Mutakabbir, JC, Stamper, KC, Morales, S, Lehman, SM, Canfield, GS, Duerkop, BA, Arias, CA & Rybak, MJ 2020, 'Bacteriophage-antibiotic combinations for enterococcus faecium with varying bacteriophage and daptomycin susceptibilities', Antimicrobial Agents and Chemotherapy, vol. 64, no. 9, e00993-20. https://doi.org/10.1128/AAC.00993-20

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abstract = "Concerns regarding increased prevalence of daptomycin (DAP)-resistant strains necessitate novel therapies for Enterococcus faecium infections. Obligately lytic bacteriophages are viruses that target, infect, and kill bacterial cells. Limited studies have evaluated phage-antibiotic combinations against E. faecium. After an initial screen of eight E. faecium strains, three strains with varying DAP/phage susceptibilities were selected for further experiments. Phage-to-strain specificity contributed to synergy with antibiotics by time-kill analyses and was associated with lower development of phage resistance.",

keywords = "Bacteriophage-antibiotic combinations, Bacteriophages, Daptomycin, Enterococcus faecium",

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AU - Stamper, Kyle C.

AU - Morales, Sandra

AU - Lehman, Susan M.

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AB - Concerns regarding increased prevalence of daptomycin (DAP)-resistant strains necessitate novel therapies for Enterococcus faecium infections. Obligately lytic bacteriophages are viruses that target, infect, and kill bacterial cells. Limited studies have evaluated phage-antibiotic combinations against E. faecium. After an initial screen of eight E. faecium strains, three strains with varying DAP/phage susceptibilities were selected for further experiments. Phage-to-strain specificity contributed to synergy with antibiotics by time-kill analyses and was associated with lower development of phage resistance.

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Bacteriophage-antibiotic combinations for enterococcus faecium with varying bacteriophage and daptomycin susceptibilities

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Keywords

ASJC Scopus subject areas

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