Bacterial translocation across ePTFE vascular graft surfaces

Supriya Narasimhan, Saima Aslam, Peter H. Lin, Carlos F. Bechara, Mohammad D. Mansouri, Rabih O. Darouiche

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objectives: Vascular graft infections arise from bacterial colonization of either the external or internal graft surfaces. We assessed whether methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli could translocate through pores of ePTFE grafts. Methods: To assess translocation from the internal to the external surface, we placed 105 cfu of bacterial suspension inside ePTFE graft segments and suspended them in sterile broth for 72 h. To assess translocation from the external to the internal surface, we placed sterile broth inside ePTFE segments, and incubated them for 72 h in a bacterial suspension (105 cfu/mL). At 72 h, in addition to culturing the sterile broth and bacterial suspensions, the external and internal surfaces were first qualitatively cultured separately and then quantitatively cultured by sonication. Results: At 72 h, the sterile broth remained sterile. The bacterial suspensions yielded 107-109 cfu/mL. Graft cultures indicated that colonization of one surface with either organism did not result in bacterial translocation to the other surface. Quantitative bacterial counts of the external vs. internal surfaces were significantly different (p < 0.01). Conclusions: MRSA and E. coli do not translocate across ePTFE graft surfaces. These in-vitro findings help elucidate the pathogenesis of graft infections and prompt conduction of validation studies in-vivo.

Original languageEnglish (US)
Pages (from-to)486-490
Number of pages5
JournalJournal of Infection
Volume60
Issue number6
DOIs
StatePublished - Jun 2010

Keywords

  • EPTFE grafts
  • Escherichia coli
  • Staphylococcus aureus
  • Translocation

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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