Avoiding ischemia reperfusion injury in liver transplantation

Yixi Zhang, Changjun Huang, Weiqiang Ju, Qiang Zhao, Maogen Chen, Linhe Wang, Dongping Wang, Linwei Wu, Zebin Zhu, Shanzhou Huang, Zhiheng Zhang, Caihui Zhu, Rongxing Xie, Yinghua Chen, Anbin Hu, Xiaofeng Zhu, Zhiyong Guo, Xiaoshun He

Research output: Contribution to journalArticlepeer-review

Abstract

Currently, ex situ machine perfusion is a burgeoning technique that provides a better preservation method for donor organs than conventional static cold preservation (0–4 °C). A continuous blood supply to organs using machine perfusion from procurement and preservation to implantation facilitates complete prevention of ischemia reperfusion injury and permits ex situ functional assessment of donor livers before transplantation. In this manuscript, we provide a step-by-step ischemia-free liver transplantation protocol in which an ex situ normothermic machine perfusion apparatus is used for pulsatile perfusion through the hepatic artery and continuous perfusion of the portal vein from human donor livers to recipients. In the perfusion period, biochemical analysis of the perfusate is conducted to assess the metabolic activity of the liver, and a liver biopsy is also performed to evaluate the degree of injury. Ischemia-free liver transplantation is a promising method to avoid ischemia-reperfusion injury and may potentially increase the donor pool for transplantation.

Original languageEnglish (US)
Article numbere61485
Pages (from-to)1-9
Number of pages9
JournalJournal of Visualized Experiments
Volume2020
Issue number166
DOIs
StatePublished - 2020

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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