Avidin-Biotin Micropatterning Methods for Biosensor Applications

Reid N. Orth, T. G. Clark, H. G. Craighead

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

High-resolution patterning methods have been developed to immobilize functional proteins onto a silicon dioxide surface for biosensor applications. Antibody lines, as small as 5 μm in width, with intervening 5 μm spacings, were patterned on oxidized silicon wafers using avidin-biotin chemistry. The N-hydroxysuccinimide (NHS) ester of photoactivatable biotin was covalently bound to a self assembled monolayer (SAM) of 3-amino-propyltriethoxysilane (3-APTS) after irradiation by 350 nm ultraviolet (UV) light from a 25 W Hg arc lamp. The patterned layers were evaluated using fluorescent imaging of Alexa-488 conjugated avidin and two fluorescence-conjugated antibodies. This technique allows binding of any biotinylated compound without exposure to harmful UV light, extreme pH, toxic chemicals, or high salinity.

Original languageEnglish (US)
Pages (from-to)29-34
Number of pages6
JournalBiomedical Microdevices
Volume5
Issue number1
DOIs
StatePublished - Mar 2003

Keywords

  • Avidin-biotin
  • Biosensor
  • Micropatterning

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Genetics
  • Neuroscience(all)

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