Autophagy proteins regulate innate immune responses by inhibiting the release of mitochondrial DNA mediated by the NALP3 inflammasome

Kiichi Nakahira, Jeffrey Adam Haspel, Vijay A.K. Rathinam, Seon Jin Lee, Tamas Dolinay, Hilaire C. Lam, Joshua A. Englert, Marlene Rabinovitch, Manuela Cernadas, Hong Pyo Kim, Katherine A. Fitzgerald, Stefan W. Ryter, Augustine M.K. Choi

Research output: Contribution to journalArticle

1639 Scopus citations

Abstract

Autophagy, a cellular process for organelle and protein turnover, regulates innate immune responses. Here we demonstrate that depletion of the autophagic proteins LC3B and beclin 1 enhanced the activation of caspase-1 and secretion of interleukin 1β (IL-1β) and IL-18. Depletion of autophagic proteins promoted the accumulation of dysfunctional mitochondria and cytosolic translocation of mitochondrial DNA (mtDNA) in response to lipopolysaccharide (LPS) and ATP in macrophages. Release of mtDNA into the cytosol depended on the NALP3 inflammasome and mitochondrial reactive oxygen species (ROS). Cytosolic mtDNA contributed to the secretion of IL-1β and IL-18 in response to LPS and ATP. LC3B-deficient mice produced more caspase-1-dependent cytokines in two sepsis models and were susceptible to LPS-induced mortality. Our study suggests that autophagic proteins regulate NALP3-dependent inflammation by preserving mitochondrial integrity.

Original languageEnglish (US)
Pages (from-to)222-230
Number of pages9
JournalNature immunology
Volume12
Issue number3
DOIs
StatePublished - Mar 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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