Automated single molecule selection

Benjamin R. Cipriany, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biological systems exhibit significant heterogeneity when studied at the single molecule level. While significant progress has been reported in the development of techniques to quantify individual molecules, preparative methods are needed to allow selected extraction of molecules for study by complementary methods. We present the development of a field programmable gate array (FPGA) system to automate the selection of individual molecules within a nanofluidic device based upon their fluorescence. Real-time signals processing is performed to identify molecules of interest and then to actuate changes in electrokinetic flow, toward collecting molecules for subsequent analysis.

Original languageEnglish (US)
Title of host publication2011 International Electron Devices Meeting, IEDM 2011
DOIs
StatePublished - Dec 1 2011
Event2011 IEEE International Electron Devices Meeting, IEDM 2011 - Washington, DC, United States
Duration: Dec 5 2011Dec 7 2011

Other

Other2011 IEEE International Electron Devices Meeting, IEDM 2011
CountryUnited States
CityWashington, DC
Period12/5/1112/7/11

Keywords

  • DNA
  • Epigenetics
  • Field Programmable Gate Array (FPGA)
  • Fluorescence Actuated Sorting
  • Matched Filter
  • Nanofluidics

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Automated single molecule selection'. Together they form a unique fingerprint.

Cite this