Automated single molecule selection

Benjamin R. Cipriany; Harold G. Craighead

doi:10.1109/IEDM.2011.6131682

Automated single molecule selection

Benjamin R. Cipriany, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Biological systems exhibit significant heterogeneity when studied at the single molecule level. While significant progress has been reported in the development of techniques to quantify individual molecules, preparative methods are needed to allow selected extraction of molecules for study by complementary methods. We present the development of a field programmable gate array (FPGA) system to automate the selection of individual molecules within a nanofluidic device based upon their fluorescence. Real-time signals processing is performed to identify molecules of interest and then to actuate changes in electrokinetic flow, toward collecting molecules for subsequent analysis.

Original language	English (US)
Title of host publication	2011 International Electron Devices Meeting, IEDM 2011
DOIs	https://doi.org/10.1109/IEDM.2011.6131682
State	Published - Dec 1 2011
Event	2011 IEEE International Electron Devices Meeting, IEDM 2011 - Washington, DC, United States Duration: Dec 5 2011 → Dec 7 2011

Other

Other	2011 IEEE International Electron Devices Meeting, IEDM 2011
Country/Territory	United States
City	Washington, DC
Period	12/5/11 → 12/7/11

Keywords

DNA
Epigenetics
Field Programmable Gate Array (FPGA)
Fluorescence Actuated Sorting
Matched Filter
Nanofluidics

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics
Electronic, Optical and Magnetic Materials
Materials Chemistry

Access to Document

10.1109/IEDM.2011.6131682

Cite this

@inproceedings{469564d81c8140edbc908746b7f3123e,

title = "Automated single molecule selection",

abstract = "Biological systems exhibit significant heterogeneity when studied at the single molecule level. While significant progress has been reported in the development of techniques to quantify individual molecules, preparative methods are needed to allow selected extraction of molecules for study by complementary methods. We present the development of a field programmable gate array (FPGA) system to automate the selection of individual molecules within a nanofluidic device based upon their fluorescence. Real-time signals processing is performed to identify molecules of interest and then to actuate changes in electrokinetic flow, toward collecting molecules for subsequent analysis.",

keywords = "DNA, Epigenetics, Field Programmable Gate Array (FPGA), Fluorescence Actuated Sorting, Matched Filter, Nanofluidics",

author = "Cipriany, {Benjamin R.} and Craighead, {Harold G.}",

year = "2011",

month = dec,

day = "1",

doi = "10.1109/IEDM.2011.6131682",

language = "English (US)",

isbn = "9781457705052",

booktitle = "2011 International Electron Devices Meeting, IEDM 2011",

note = "2011 IEEE International Electron Devices Meeting, IEDM 2011 ; Conference date: 05-12-2011 Through 07-12-2011",

}

TY - GEN

T1 - Automated single molecule selection

AU - Cipriany, Benjamin R.

AU - Craighead, Harold G.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Biological systems exhibit significant heterogeneity when studied at the single molecule level. While significant progress has been reported in the development of techniques to quantify individual molecules, preparative methods are needed to allow selected extraction of molecules for study by complementary methods. We present the development of a field programmable gate array (FPGA) system to automate the selection of individual molecules within a nanofluidic device based upon their fluorescence. Real-time signals processing is performed to identify molecules of interest and then to actuate changes in electrokinetic flow, toward collecting molecules for subsequent analysis.

AB - Biological systems exhibit significant heterogeneity when studied at the single molecule level. While significant progress has been reported in the development of techniques to quantify individual molecules, preparative methods are needed to allow selected extraction of molecules for study by complementary methods. We present the development of a field programmable gate array (FPGA) system to automate the selection of individual molecules within a nanofluidic device based upon their fluorescence. Real-time signals processing is performed to identify molecules of interest and then to actuate changes in electrokinetic flow, toward collecting molecules for subsequent analysis.

KW - DNA

KW - Epigenetics

KW - Field Programmable Gate Array (FPGA)

KW - Fluorescence Actuated Sorting

KW - Matched Filter

KW - Nanofluidics

UR - http://www.scopus.com/inward/record.url?scp=84856979353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856979353&partnerID=8YFLogxK

U2 - 10.1109/IEDM.2011.6131682

DO - 10.1109/IEDM.2011.6131682

M3 - Conference contribution

AN - SCOPUS:84856979353

SN - 9781457705052

BT - 2011 International Electron Devices Meeting, IEDM 2011

T2 - 2011 IEEE International Electron Devices Meeting, IEDM 2011

Y2 - 5 December 2011 through 7 December 2011

ER -

Automated single molecule selection

Abstract

Other

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this