TY - JOUR
T1 - Automated analysis of zebrafish images for screening toxicants
AU - Hans, Charu
AU - McCollum, Catherine W.
AU - Bondesson, Maria B.
AU - Gustafsson, Jan Ake
AU - Shah, Shishir K.
AU - Merchant, Fatima A.
PY - 2013
Y1 - 2013
N2 - An important factor facilitating the application of zebrafish in biomedical research is high throughput screening of vertebrate animal models. For example, being able to model the growth of blood vessel in the vasculature system is interesting for understanding both the circulatory system in humans, and for facilitating large scale screening of the influence of various chemicals on vascular development. Compared to other models, the zebrafish embryo is an attractive alternative for environmental risk assessment of chemicals since it offers the possibility to perform high-throughput analyses in vivo. However the lack of an automated image analysis framework restricts high throughput screening. In this paper, we provide a method for quantitative measurements of zebrafish blood vessel morphology since it is difficult to assess changes in vessel structure by visual inspection. The method presented is generalized, i.e. it is not restricted to any specific chemically treated zebrafish, and can be used with wide variety of chemicals.
AB - An important factor facilitating the application of zebrafish in biomedical research is high throughput screening of vertebrate animal models. For example, being able to model the growth of blood vessel in the vasculature system is interesting for understanding both the circulatory system in humans, and for facilitating large scale screening of the influence of various chemicals on vascular development. Compared to other models, the zebrafish embryo is an attractive alternative for environmental risk assessment of chemicals since it offers the possibility to perform high-throughput analyses in vivo. However the lack of an automated image analysis framework restricts high throughput screening. In this paper, we provide a method for quantitative measurements of zebrafish blood vessel morphology since it is difficult to assess changes in vessel structure by visual inspection. The method presented is generalized, i.e. it is not restricted to any specific chemically treated zebrafish, and can be used with wide variety of chemicals.
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U2 - 10.1109/EMBC.2013.6610172
DO - 10.1109/EMBC.2013.6610172
M3 - Article
C2 - 24110359
SN - 1557-170X
VL - 2013
SP - 3004
EP - 3007
JO - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
JF - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
ER -