Attenuation of hyperoxic lung injury by the CYP1A inducer β-naphthoflavone

Anuj Sinha, Kathirvel Muthiah, Weiwu Jiang, Xanthi Couroucli, Roberto Barrios, Bhagavatula Moorthy

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Supplemental oxygen, frequently used in premature infants, has been implicated in the development of bronchopulmonary dysplasia (BPD). While the mechanisms of oxygen-induced lung injury are not known, reactive oxygen species (ROS) are most likely involved in the process. Here, we tested the hypothesis that upregulation of cytochrome P450 (CYP) 1A isoforms in lung and liver may lead to protection against hyperoxic lung injury. Adult male Sprague-Dawley rats were pretreated with the CYP1A inducer beta-naphthoflavone (β-NF) (80 mg/kg/day), once daily for 4 days, followed by exposure to hyperoxic environment (O2 > 95%) or room air (normoxia) for 60 h. Pleural effusions were measured as estimates of lung injury. Activities of hepatic and pulmonary CYP1A1 were determined by measurement of ethoxyresorufin O-deethylation (EROD) activity. Northern hybridization and Western blot analysis of lung and liver were performed to assess mRNA and protein levels, respectively. Our results showed that β-NF-treated animals, which displayed the highest pulmonary and hepatic induction in EROD activity (10-fold and 8-fold increase over corn oil (CO) controls, respectively), offered the most protective effect against hyperoxic lung injury, p < 0.05. Northern and Western blot analysis correlated well with enzyme activities. Our results showed an inverse correlation between pulmonary and hepatic CYP1A expression and the extent of lung injury, which supports the hypothesis that CYP1A enzyme plays a protective role against oxygen-mediated tissue damage.

Original languageEnglish (US)
Pages (from-to)204-212
Number of pages9
JournalToxicological Sciences
Volume87
Issue number1
DOIs
StatePublished - Sep 2005

Keywords

  • Beta-naphthoflavone
  • Cytochrome P450
  • Hyperoxia

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Attenuation of hyperoxic lung injury by the CYP1A inducer β-naphthoflavone'. Together they form a unique fingerprint.

Cite this