Attachment of rat hepatocytes to collagen and fibronectin; A study using antibodies directed against cell surface components

Kristofer Rubin; Staffan Johansson; Inger Pettersson; Carin Ocklind; Björn Öbrink; Magnus Höök

doi:10.1016/0006-291X(79)90586-2

Attachment of rat hepatocytes to collagen and fibronectin; A study using antibodies directed against cell surface components

Kristofer Rubin, Staffan Johansson, Inger Pettersson, Carin Ocklind, Björn Öbrink, Magnus Höök

Research Institute

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46 Scopus citations

Abstract

Attachment of rat hepatocytes to collagen but not to fibronectin substrata was efficiently inhibited by antibodies against the hepatocyte surface. Further analyses of this inhibition suggested that hepatocyte attachment to collagen involves cell surface antigens which are not identical to membrane bound fibronectin or collagen. Rabbit antibodies against rat fibronectin inhibited hepatocyte attachment to rat fibronectin but not to collagen or rabbit fibronectin. After plasmin digestion of fibronectin, peptides were isolated that lacked affinity for collagen but could serve as a substratum for hepatocyte attachment. These results suggested that attachment to fibronectin does not involve membrane bound fibronectin or collagen.

Original language	English (US)
Pages (from-to)	86-94
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(79)90586-2
State	Published - Nov 14 1979

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(79)90586-2

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title = "Attachment of rat hepatocytes to collagen and fibronectin; A study using antibodies directed against cell surface components",

abstract = "Attachment of rat hepatocytes to collagen but not to fibronectin substrata was efficiently inhibited by antibodies against the hepatocyte surface. Further analyses of this inhibition suggested that hepatocyte attachment to collagen involves cell surface antigens which are not identical to membrane bound fibronectin or collagen. Rabbit antibodies against rat fibronectin inhibited hepatocyte attachment to rat fibronectin but not to collagen or rabbit fibronectin. After plasmin digestion of fibronectin, peptides were isolated that lacked affinity for collagen but could serve as a substratum for hepatocyte attachment. These results suggested that attachment to fibronectin does not involve membrane bound fibronectin or collagen.",

author = "Kristofer Rubin and Staffan Johansson and Inger Pettersson and Carin Ocklind and Bj{\"o}rn {\"O}brink and Magnus H{\"o}{\"o}k",

note = "Funding Information: This investigation was supported by grants from the Swedish Medical Research Council (projects No 03X-4, 05200, 05197, 02309), Gustaf V:s 80-drsfond, Svenska livf6rslkringsbalagens nHmnd fir medicinsk farskning, EIR:s 50-grsstiftelse and the Medical Faculty, University Uppsala, Sweden. The expert technical assistance by MS Eva Andersson gratefully acknowledged. Part of this work was presented at the VIth Meeting of the Federation of the European Connective Tissue Clubs, CrPteil, France, August 1978. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

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doi = "10.1016/0006-291X(79)90586-2",

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AU - Rubin, Kristofer

AU - Johansson, Staffan

AU - Pettersson, Inger

AU - Ocklind, Carin

AU - Öbrink, Björn

AU - Höök, Magnus

N1 - Funding Information: This investigation was supported by grants from the Swedish Medical Research Council (projects No 03X-4, 05200, 05197, 02309), Gustaf V:s 80-drsfond, Svenska livf6rslkringsbalagens nHmnd fir medicinsk farskning, EIR:s 50-grsstiftelse and the Medical Faculty, University Uppsala, Sweden. The expert technical assistance by MS Eva Andersson gratefully acknowledged. Part of this work was presented at the VIth Meeting of the Federation of the European Connective Tissue Clubs, CrPteil, France, August 1978. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1979/11/14

Y1 - 1979/11/14

N2 - Attachment of rat hepatocytes to collagen but not to fibronectin substrata was efficiently inhibited by antibodies against the hepatocyte surface. Further analyses of this inhibition suggested that hepatocyte attachment to collagen involves cell surface antigens which are not identical to membrane bound fibronectin or collagen. Rabbit antibodies against rat fibronectin inhibited hepatocyte attachment to rat fibronectin but not to collagen or rabbit fibronectin. After plasmin digestion of fibronectin, peptides were isolated that lacked affinity for collagen but could serve as a substratum for hepatocyte attachment. These results suggested that attachment to fibronectin does not involve membrane bound fibronectin or collagen.

AB - Attachment of rat hepatocytes to collagen but not to fibronectin substrata was efficiently inhibited by antibodies against the hepatocyte surface. Further analyses of this inhibition suggested that hepatocyte attachment to collagen involves cell surface antigens which are not identical to membrane bound fibronectin or collagen. Rabbit antibodies against rat fibronectin inhibited hepatocyte attachment to rat fibronectin but not to collagen or rabbit fibronectin. After plasmin digestion of fibronectin, peptides were isolated that lacked affinity for collagen but could serve as a substratum for hepatocyte attachment. These results suggested that attachment to fibronectin does not involve membrane bound fibronectin or collagen.

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Attachment of rat hepatocytes to collagen and fibronectin; A study using antibodies directed against cell surface components

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