Attachment of rat hepatocytes to collagen and fibronectin; A study using antibodies directed against cell surface components

Kristofer Rubin, Staffan Johansson, Inger Pettersson, Carin Ocklind, Björn Öbrink, Magnus Höök

    Research output: Contribution to journalArticlepeer-review

    46 Scopus citations

    Abstract

    Attachment of rat hepatocytes to collagen but not to fibronectin substrata was efficiently inhibited by antibodies against the hepatocyte surface. Further analyses of this inhibition suggested that hepatocyte attachment to collagen involves cell surface antigens which are not identical to membrane bound fibronectin or collagen. Rabbit antibodies against rat fibronectin inhibited hepatocyte attachment to rat fibronectin but not to collagen or rabbit fibronectin. After plasmin digestion of fibronectin, peptides were isolated that lacked affinity for collagen but could serve as a substratum for hepatocyte attachment. These results suggested that attachment to fibronectin does not involve membrane bound fibronectin or collagen.

    Original languageEnglish (US)
    Pages (from-to)86-94
    Number of pages9
    JournalBiochemical and Biophysical Research Communications
    Volume91
    Issue number1
    DOIs
    StatePublished - Nov 14 1979

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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