Attaching single biomolecules selectively to the apex of AFM tips for measuring specific interactions

Jianhua Gu, Zhongdang Xiao, Chi Ming Yam, Guoting Qin, Maxence Deluge, Sabine Boutet, Chengzhi Cai

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We present a general approach for preparing well-defined AFM tips for probing single target molecules. We demonstrated that carboxylic acid groups could be generated by electrochemical oxidation selectively at the apex of an AFM tip that is coated with a monolayer of oligo(ethylene glycol) derivatives for resisting nonspecific interactions. These carboxylic acid groups were used as handles to tether only one ligand molecule, such as biotin, to the tip apex for measurement of specific interactions with biomolecules.

Original languageEnglish (US)
Pages (from-to)L31-L33
JournalBiophysical Journal
Volume89
Issue number5
DOIs
StatePublished - Nov 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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