Attaching single biomolecules selectively to the apex of AFM tips for measuring specific interactions

Jianhua Gu; Zhongdang Xiao; Chi Ming Yam; Guoting Qin; Maxence Deluge; Sabine Boutet; Chengzhi Cai

doi:10.1529/biophysj.105.069104

Attaching single biomolecules selectively to the apex of AFM tips for measuring specific interactions

Jianhua Gu, Zhongdang Xiao, Chi Ming Yam, Guoting Qin, Maxence Deluge, Sabine Boutet, Chengzhi Cai

Houston Methodist

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We present a general approach for preparing well-defined AFM tips for probing single target molecules. We demonstrated that carboxylic acid groups could be generated by electrochemical oxidation selectively at the apex of an AFM tip that is coated with a monolayer of oligo(ethylene glycol) derivatives for resisting nonspecific interactions. These carboxylic acid groups were used as handles to tether only one ligand molecule, such as biotin, to the tip apex for measurement of specific interactions with biomolecules.

Original language	English (US)
Pages (from-to)	L31-L33
Journal	Biophysical Journal
Volume	89
Issue number	5
DOIs	https://doi.org/10.1529/biophysj.105.069104
State	Published - Nov 2005

ASJC Scopus subject areas

Biophysics

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10.1529/biophysj.105.069104

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AU - Gu, Jianhua

AU - Xiao, Zhongdang

AU - Yam, Chi Ming

AU - Qin, Guoting

AU - Deluge, Maxence

AU - Boutet, Sabine

AU - Cai, Chengzhi

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AB - We present a general approach for preparing well-defined AFM tips for probing single target molecules. We demonstrated that carboxylic acid groups could be generated by electrochemical oxidation selectively at the apex of an AFM tip that is coated with a monolayer of oligo(ethylene glycol) derivatives for resisting nonspecific interactions. These carboxylic acid groups were used as handles to tether only one ligand molecule, such as biotin, to the tip apex for measurement of specific interactions with biomolecules.

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Attaching single biomolecules selectively to the apex of AFM tips for measuring specific interactions

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