Ataxia-telangiectasia: Chronic activation of damage-responsive functions is reduced by α-lipoic acid

Magtouf Gatei, Dganit Shkedy, Kum Kum Khanna, Tamar Uziel, Yosef Shiloh, Tej K. Pandita, Martin F. Lavin, Galit Rotman

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Cells from patients with the genetic disorder ataxia-telangiectasia (A-T) are hypersensitive to ionizing radiation and radiomimetic agents, both of which generate reactive oxygen species capable of causing oxidative damage to DNA and other macromolecules. We describe in A-T cells constitutive activation of pathways that normally respond to genotoxic stress. Basal levels of p53 and p21WAF1/CIP1, phosphorylation on serine 15 of p53, and the Tyr15-phosphorylated form of cdc2 are chronically elevated in these cells. Treatment of A-T cells with the antioxidant α-lipoic acid significantly reduced the levels of these proteins, pointing to the involvement of reactive oxygen species in their chronic activation. These findings suggest that the absence of functional ATM results in a mild but continuous state of oxidative stress, which could account for several features of the pleiotropic phenotype of A-T.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalOncogene
Volume20
Issue number3
DOIs
StatePublished - Jan 18 2001

Keywords

  • α-lipoic acid
  • Ataxia-Telangiectasia
  • ATM
  • cdc2
  • Oxidative stress
  • p21
  • p53

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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