Asthma, rhinitis, other respiratory diseases: Proliferation and release of IL-5 and IFN-γ by peripheral blood mononuclear cells from cat-allergic asthmatics and rhinitics, non-cat-allergic asthmatics, and normal controls to peptides derived from Fel d 1 chain 1

Brigitte M. Haselden, Ekaterina Syrigou, Meinir Jones, David Huston, Kunio Ichikawa, Martin D. Chapman, A. Barry Kay, Mark Larché

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Background: In general, T cells from normal, nonatopic individuals respond to aeroallergens with synthesis and release of IFN-γ. In contrast, release of T H2-type cytokines by activated lymphocytes is a feature of allergic rhinitis and atopic asthma. Objective: The purpose of this study was to determine differences in T-cell recognition of epitopes within allergenic sequences, in terms of proliferation and cytokine production, in subjects with atopic asthma compared with subjects with allergic rhinitis and normal controls. Methods: Proliferative responses and IL-S/IFN-γ release patterns from PBMCs from cat-allergic asthmatic, cat-allergic rhinitic, and non-cat-allergic asthmatic subjects and nonatopic normal controls were determined in primary cultures. Cells were challenged with 7 overlapping peptides spanning chain 1 of the major cat allergen, Fel d I. Results: The 4 groups did not differ with respect to the ability to mount proliferative responses to Fel d 1 peptides. In all groups, the IFN-γ responses were predominantly to the amino terminus peptides. Cat-allergic and non-cat-allergic asthmatic subjects (and not cat-allergic rhinitic subjects and normal controls) made IL-5 responses to most of the Fel d I peptides, the result being a mixed (T H0) cytokine response at the N-terminus and a restricted (T H2) response at the C-terminus. Conclusion: Proliferative and IL-5/IFN-γ responses of T cells from asthmatic and atopic rhinitic subjects and normal controls to allergen peptides can be dissociated. Furthermore, differing cytokine responses to peptides derived from a single antigen suggest that certain domains of the molecule might preferentially induce IL-5 rather than IFN-γ and as a result could be more important in disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)349-356
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume108
Issue number3
DOIs
StatePublished - Sep 2001

Keywords

  • Allergy
  • Asthma
  • Cytokine
  • Peptide
  • T lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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