Association of genetic polymorphism of glutathione S-transferase M1, T1, P1 and susceptibility to bladder cancer

D. S L Srivastava, Dhruva Kumar Mishra, Anil Mandhani, Balraj Mittal, Anant Kumar, Rama Devi Mittal

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Objective: Glutathione-S-transferases (GSTs) are active in the detoxification of wide variety of endogenous or exogenous carcinogens. We examined the association of the GST gene polymorphism with sporadic bladder cancer patients in Northern India. Material and methods: The study constituted of 106 bladder cancer cases and 370 age-matched controls. The GSTT1 and GSTM1 null genotypes were identified by multiplex PCR and GSTP1313 A/G by Polymerase Chain Reaction/Restriction Fragment Length Polymorphism method (PCR/RFLP). Results: We observed non-significant association in null alleles of the GSTM1 (p = 0.611, OR = 1.12, 95% CI = 0.72-1.74 and GSTT1 (p = 0.135, OR = 1.45, 95% CI = 0.89-2.37) with risk of bladder cancer. However, the G/G genotype of the GSTP1 gene polymorphism was highly significant when compared to controls (p = 0.000, OR = 7.12, 95% CI = 3.14-16.16). The combined analysis of the three risk genotypes demonstrated further increase in the risk of bladder cancer (p = 0.000, OR = 7.29 95% CI = 2.81-18.93). Conclusion: Our study demonstrated that GSTP1313 G/G polymorphism is a strong predisposing risk factor for bladder cancer. Combination of three GST genotypes association exhibiting gene-gene interaction further substantiates the increased risk of bladder cancer.

Original languageEnglish (US)
Pages (from-to)339-344
Number of pages6
JournalEuropean Urology
Volume48
Issue number2
DOIs
StatePublished - Aug 2005

Keywords

  • Bladder cancer
  • Detoxification
  • Gene-gene interaction
  • Genetic polymorphism
  • Glutathione S-transferase

ASJC Scopus subject areas

  • Urology

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