Assignment of the βb1 crystallin gene (CRYBB1) to human chromosome 22 and mouse chromosome 5

T. J.M. Hulsebos, D. J. Gilbert, O. Delattre, L. J. Smink, I. Dunham, A. Westerveld, G. Thomas, Nancy A. Jenkins, Neal G. Copeland

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

By using primers complementary to the rat βB1 crystallin gene sequence, we amplified exons 5 and 6 of the orthologous human gene (CRYBB1). The amplified human segments displayed greater than 88% sequence homology to the corresponding rat and bovine sequences. CRYBB1 was assigned to the group 5 region in 22q11.2-q12.1 by hybridizing the exon 6 PCR product to somatic cell hybrids containing defined portions of human chromosome 22. The exon 5 and exon 6 PCR products of CRYBB1 were used to localize, by interspecific backcross mapping, the mouse gene (Crybb1) to the central portion of chromosome 5. Three other β crystallin genes (βB2(1), βB3, and βA4) have previously been mapped to the same regions in human and mouse. We demonstrate that the/βB1 and βA4 crystallin genes are very closely linked in the two species. These assignments complete the mapping and identification of the human and mouse homologues of the major β crystallins genes that are expressed in the bovine lens.

Original languageEnglish (US)
Pages (from-to)712-718
Number of pages7
JournalGenomics
Volume29
Issue number3
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Genetics

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