Assessment of chemical species of lead accumulated in tidemarks of human articular cartilage by X-ray absorption near-edge structure analysis

Florian Meirer, Bernhard Pemmer, Giancarlo Pepponi, Norbert Zoeger, Peter Wobrauschek, Simone Sprio, Anna Tampieri, Joerg Goettlicher, Ralph Steininger, Stefan Mangold, Paul Roschger, Andrea Berzlanovich, Jochen G. Hofstaetter, Christina Streli

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A highly specific accumulation of the toxic element lead was recently measured in the transition zone between non-calcified and calcified normal human articular cartilage. This transition zone, the so-called tidemark, is considered to be an active calcification front of great clinical importance. However, little is known about the mechanisms of accumulation and the chemical form of Pb in calcified cartilage and bone. Using spatially resolved X-ray absorption near-edge structure analysis (μ-XANES) at the Pb L 3-edge, the chemical state of Pb in the osteochondral region was investigated. The feasibility of the μ-XANES set-up at the SUL-X beamline (ANKA synchrotron light source) was tested and confirmed by comparing XANES spectra of bulk Pb-reference compounds recorded at both the XAS and the SUL-X beamline at ANKA. The μ-XANES set-up was then used to investigate the tidemark region of human bone (two patella samples and one femoral head sample). The spectra recorded at the tidemark and at the trabecular bone were found to be highly correlated with the spectra of synthetic Pb-doped carbonated hydroxyapatite, suggesting that in both of these very different tissues Pb is incorporated into the hydroxyapatite structure.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalJournal of Synchrotron Radiation
Volume18
Issue number2
DOIs
StatePublished - Mar 2011

Keywords

  • human bone
  • Pb L-edge XANES
  • tidemark
  • trabecular bone
  • X-ray absorption spectroscopy

ASJC Scopus subject areas

  • Instrumentation
  • Nuclear and High Energy Physics
  • Radiation

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