Aspergillus fumigatus hyphal damage caused by noninvasive radiofrequency field-induced hyperthermia

Warna D. Kaluarachchi, Brandon T. Cisneros, Stuart J. Corr, Nathaniel D. Albert, Steven A. Curley, Dimitrios P. Kontoyiannis

Research output: Contribution to journalArticlepeer-review

Abstract

We studied the effect of noninvasive radiofrequency-induced hyperthermia on the viability of Aspergillus fumigatus hyphae in vitro. Radiofrequency-induced hyperthermia resulted in significant (>70%, P<0.0001) hyphal damage in a time and thermal dose-dependent fashion as assessed by XTT [(sodium 2,3,-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl] (1)-2H-tetrazolium inner salt)], DiBAC [bis-(1,3-dibutylbarbituric acid) trimethine oxonol] staining, and transmission electron microscopy. For comparison, water bath hyperthermia was used over the range of 45 to 55°C to study hyphal damage. Radiofrequency-induced hyperthermia resulted in severe damage to the outer fibrillar layer of hyphae at a shorter treatment time compared to water bath hyperthermia. Our preliminary data suggest that radiofrequency-induced hyperthermia might be an additional therapeutic approach to use in the management of mold infections.

Original languageEnglish (US)
Pages (from-to)4444-4448
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume57
Issue number9
DOIs
StatePublished - Sep 2013

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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