Aryl hydrocarbon hydroxylase (AHH) induction by polybrominated biphenyls (PBBs): Enhancement by photolysis

Larry W. Robertson; Andrew Parkinson; Brock Chittim; Stelvio Bandiera; Thomas W. Sawyer; Stephen Safe

doi:10.1016/0300-483X(81)90110-4

Aryl hydrocarbon hydroxylase (AHH) induction by polybrominated biphenyls (PBBs): Enhancement by photolysis

Larry W. Robertson, Andrew Parkinson, Brock Chittim, Stelvio Bandiera, Thomas W. Sawyer, Stephen Safe

Research output: Contribution to journal › Article › peer-review

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Abstract

Irradiation of the commercial polybrominated biphenyl (PBB) mixture, fireMaster BP-6, in cyclohexane solution at 300 nm for 930 min resulted in a marked diminution of the major components of the mixture. Administration of the photolyzed PBB mixture or fireMaster BP-6 to immature mall Wistar rats caused dose-related decreases in thymus weight and increases in hepatic microsomal benzo[a]pyrene hydroxylase (AHH), 4-dimethylaminoantypyrine N-demethylase and NADPH-cytochrome c reductase activities and cytochrome P-450 content. The dose effecting half-maximal AHH induction for the photolyzed PBBs (9 mg · kg^-1) was approximately 6 times lower than that of fireMaster Bp-6 (50 mg · kg^-1). Futhermore, the concentration of photolyzed PBBs (2 μM) required to displace 50% of the specifically-bound [³H]TCDD from its high-affinity cytosolic Ah receptor was approximately 150 times lower than that required for fireMaster BP-6 (300 μM), as measured by sucrose density gradient centrifugation analysis. The results suggest that the photolysis of the commercial PBB mixture yields products which possess increased biologic activity.

Original language	English (US)
Pages (from-to)	103-114
Number of pages	12
Journal	Toxicology
Volume	22
Issue number	2
DOIs	https://doi.org/10.1016/0300-483X(81)90110-4
State	Published - 1981

ASJC Scopus subject areas

Toxicology

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10.1016/0300-483X(81)90110-4

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@article{18c7c96e0936414d90867b82b7f21cd8,

title = "Aryl hydrocarbon hydroxylase (AHH) induction by polybrominated biphenyls (PBBs): Enhancement by photolysis",

abstract = "Irradiation of the commercial polybrominated biphenyl (PBB) mixture, fireMaster BP-6, in cyclohexane solution at 300 nm for 930 min resulted in a marked diminution of the major components of the mixture. Administration of the photolyzed PBB mixture or fireMaster BP-6 to immature mall Wistar rats caused dose-related decreases in thymus weight and increases in hepatic microsomal benzo[a]pyrene hydroxylase (AHH), 4-dimethylaminoantypyrine N-demethylase and NADPH-cytochrome c reductase activities and cytochrome P-450 content. The dose effecting half-maximal AHH induction for the photolyzed PBBs (9 mg · kg-1) was approximately 6 times lower than that of fireMaster Bp-6 (50 mg · kg-1). Futhermore, the concentration of photolyzed PBBs (2 μM) required to displace 50% of the specifically-bound [3H]TCDD from its high-affinity cytosolic Ah receptor was approximately 150 times lower than that required for fireMaster BP-6 (300 μM), as measured by sucrose density gradient centrifugation analysis. The results suggest that the photolysis of the commercial PBB mixture yields products which possess increased biologic activity.",

author = "Robertson, {Larry W.} and Andrew Parkinson and Brock Chittim and Stelvio Bandiera and Sawyer, {Thomas W.} and Stephen Safe",

note = "Funding Information: The authors wish to express their appreciation to Dr. A.B. Okey for his advice and assistance with the receptor binding studies, Mr. S. MacKinnon and SCIEX Inc. for MS analysis and Dr. M. Mullin for capillary GC analysis of fireMaster BP-6 and the photolyzed PBBs. The financial assistance of the Research Programs Directorate, Health and Welfare Canada (606-1444-X), the National Cancer Institute (5 R01-CA21814-02), the Natural ~ciences and Engineering Research Council of Canada and the U.S. Environmental Protection Agency (CR 806-928010} is gratefully acknowledged. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1981",

doi = "10.1016/0300-483X(81)90110-4",

language = "English (US)",

volume = "22",

pages = "103--114",

journal = "Toxicology",

issn = "0300-483X",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Aryl hydrocarbon hydroxylase (AHH) induction by polybrominated biphenyls (PBBs)

T2 - Enhancement by photolysis

AU - Robertson, Larry W.

AU - Parkinson, Andrew

AU - Chittim, Brock

AU - Bandiera, Stelvio

AU - Sawyer, Thomas W.

AU - Safe, Stephen

N1 - Funding Information: The authors wish to express their appreciation to Dr. A.B. Okey for his advice and assistance with the receptor binding studies, Mr. S. MacKinnon and SCIEX Inc. for MS analysis and Dr. M. Mullin for capillary GC analysis of fireMaster BP-6 and the photolyzed PBBs. The financial assistance of the Research Programs Directorate, Health and Welfare Canada (606-1444-X), the National Cancer Institute (5 R01-CA21814-02), the Natural ~ciences and Engineering Research Council of Canada and the U.S. Environmental Protection Agency (CR 806-928010} is gratefully acknowledged. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1981

Y1 - 1981

N2 - Irradiation of the commercial polybrominated biphenyl (PBB) mixture, fireMaster BP-6, in cyclohexane solution at 300 nm for 930 min resulted in a marked diminution of the major components of the mixture. Administration of the photolyzed PBB mixture or fireMaster BP-6 to immature mall Wistar rats caused dose-related decreases in thymus weight and increases in hepatic microsomal benzo[a]pyrene hydroxylase (AHH), 4-dimethylaminoantypyrine N-demethylase and NADPH-cytochrome c reductase activities and cytochrome P-450 content. The dose effecting half-maximal AHH induction for the photolyzed PBBs (9 mg · kg-1) was approximately 6 times lower than that of fireMaster Bp-6 (50 mg · kg-1). Futhermore, the concentration of photolyzed PBBs (2 μM) required to displace 50% of the specifically-bound [3H]TCDD from its high-affinity cytosolic Ah receptor was approximately 150 times lower than that required for fireMaster BP-6 (300 μM), as measured by sucrose density gradient centrifugation analysis. The results suggest that the photolysis of the commercial PBB mixture yields products which possess increased biologic activity.

AB - Irradiation of the commercial polybrominated biphenyl (PBB) mixture, fireMaster BP-6, in cyclohexane solution at 300 nm for 930 min resulted in a marked diminution of the major components of the mixture. Administration of the photolyzed PBB mixture or fireMaster BP-6 to immature mall Wistar rats caused dose-related decreases in thymus weight and increases in hepatic microsomal benzo[a]pyrene hydroxylase (AHH), 4-dimethylaminoantypyrine N-demethylase and NADPH-cytochrome c reductase activities and cytochrome P-450 content. The dose effecting half-maximal AHH induction for the photolyzed PBBs (9 mg · kg-1) was approximately 6 times lower than that of fireMaster Bp-6 (50 mg · kg-1). Futhermore, the concentration of photolyzed PBBs (2 μM) required to displace 50% of the specifically-bound [3H]TCDD from its high-affinity cytosolic Ah receptor was approximately 150 times lower than that required for fireMaster BP-6 (300 μM), as measured by sucrose density gradient centrifugation analysis. The results suggest that the photolysis of the commercial PBB mixture yields products which possess increased biologic activity.

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U2 - 10.1016/0300-483X(81)90110-4

DO - 10.1016/0300-483X(81)90110-4

M3 - Article

C2 - 6275573

AN - SCOPUS:0019849668

SN - 0300-483X

VL - 22

SP - 103

EP - 114

JO - Toxicology

JF - Toxicology

IS - 2

ER -

Aryl hydrocarbon hydroxylase (AHH) induction by polybrominated biphenyls (PBBs): Enhancement by photolysis

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