TY - JOUR
T1 - Artifact formation during incubation of neutral steroids with commercial β-glucuronidase preparations
AU - Manson, Maureen E.
AU - Nocke-Fink, Lieselotte
AU - Gustafsson, J. Å
AU - Shackleton, C. H.I.
N1 - Funding Information:
We wish to thank Dr. R. Blowers and Mr. S. J. Line for culturing the bacterial powder and incubating media for micro organisms. The mass-spectrometric identifications were carried out with the financial support of the Swedish M.R.C. (Grant No. 13S-2S19). M. E. Manson wishes to acknowledge that results of research work reported in this communication will be incorporated in a thesis for a Council of National Academic Awards degree.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1972/4
Y1 - 1972/4
N2 - A preparation of bacterial β-glucuronidase caused the formation of artifacts when incubated with neutral steroids unless bacteria were effectively removed by filtration or suppressed by antibiotics or the addition of chloroform. For example, over 80% of 3α-hydroxy-5α-androstan-17-one substrate was converted to 5α-androstane-3,17-dione and 3β-hydroxy-5α-androstan-17-one by the bacterial preparation during a 72-h incubation. The steroid artifacts formed were characterised by combined gas chromatography-mass spectrometry.
AB - A preparation of bacterial β-glucuronidase caused the formation of artifacts when incubated with neutral steroids unless bacteria were effectively removed by filtration or suppressed by antibiotics or the addition of chloroform. For example, over 80% of 3α-hydroxy-5α-androstan-17-one substrate was converted to 5α-androstane-3,17-dione and 3β-hydroxy-5α-androstan-17-one by the bacterial preparation during a 72-h incubation. The steroid artifacts formed were characterised by combined gas chromatography-mass spectrometry.
UR - http://www.scopus.com/inward/record.url?scp=0015318960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015318960&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(72)90206-9
DO - 10.1016/0009-8981(72)90206-9
M3 - Article
C2 - 4260630
AN - SCOPUS:0015318960
VL - 38
SP - 45
EP - 49
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 1
ER -