Artifact formation during incubation of neutral steroids with commercial β-glucuronidase preparations

Maureen E. Manson, Lieselotte Nocke-Fink, J. Å Gustafsson, C. H.I. Shackleton

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A preparation of bacterial β-glucuronidase caused the formation of artifacts when incubated with neutral steroids unless bacteria were effectively removed by filtration or suppressed by antibiotics or the addition of chloroform. For example, over 80% of 3α-hydroxy-5α-androstan-17-one substrate was converted to 5α-androstane-3,17-dione and 3β-hydroxy-5α-androstan-17-one by the bacterial preparation during a 72-h incubation. The steroid artifacts formed were characterised by combined gas chromatography-mass spectrometry.

Original languageEnglish (US)
Pages (from-to)45-49
Number of pages5
JournalClinica Chimica Acta
Volume38
Issue number1
DOIs
StatePublished - Apr 1972

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Artifact formation during incubation of neutral steroids with commercial β-glucuronidase preparations'. Together they form a unique fingerprint.

Cite this