@article{e9f71d561873462389aa738cfe8a9b0b,
title = "ARNT-dependent CCR8 reprogrammed LDH isoform expression correlates with poor clinical outcomes of prostate cancer",
abstract = "Lactate dehydrogenase isozyme (LDH) is a tetramer constituted of two isoforms, LDHA and LDHB, the expression of which is associated with cell metabolism and cancer progression. Our previous study reveals that CC-chemokine ligand-18 (CCL18) is involved in progression of prostate cancer (PCa).This study aims to investigate how CCL18 regulates LDH isoform expression, and therefore, contributes to PCa progression. The data revealed that the expression of LDHA was upregulated and LDHB was downregulated in PCa cells by CCL18 at both messenger RNA and protein levels. The depletion of CCR8 reduced the ability of CCL18 to promote the proliferation, migration, and lactate production of PCa cells. Depletion of a CCR8 regulated transcription factor, ARNT, significantly reduced the expression of LDHA. In addition, The Cancer Genome Atlas dataset analyses revealed a positive correlation between CCR8 and ARNT expression. Two dimension difference gel electrophoresis revealed that the LDHA/LDHB ratio was increased in the prostatic fluid of patients with PCa and PCa tissues. Furthermore, increased LDHA/LDHB ratio was associated with poor clinical outcomes of patients with PCa. Together, our results indicate that the CCR8 pathway programs LDH isoform expression in an ARNT dependent manner and that the ratio of LDHA/LDHB has the potential to serve as biomarkers for PCa diagnosis and prognosis.",
keywords = "LDHA/LDHB ratio, CCR8, ARNT, lactate dehydrogenase, prostate cancer, Prostatic Neoplasms/genetics, Cell Proliferation, Prognosis, Chemokines, CC/genetics, Isoenzymes, Humans, Gene Expression Regulation, Neoplastic, Male, Survival Rate, Receptors, CCR8/genetics, Biomarkers, Tumor/genetics, L-Lactate Dehydrogenase/genetics, Aryl Hydrocarbon Receptor Nuclear Translocator/genetics, Tumor Cells, Cultured, Apoptosis",
author = "Guo Chen and Cai, {Zhi duan} and Lin, {Zhuo yuan} and Cong Wang and Liang, {Yu xiang} and Han, {Zhao dong} and He, {Hui chan} and Mo, {Ru jun} and Lu, {Jian ming} and Bin Pan and Wu, {Chin lee} and Fen Wang and Zhong, {Wei de}",
note = "Funding Information: This study was supported by grants from Guangzhou Municipal Science and Technology Project (201803040001), National Natural Science Foundation of China (81802567, 81802555, 815714278160062081470983), the Fundamental Research Funds for the Central Universities(21618306), Guangzhou Municipal Science and Technology Project (201803040001, 201710010081, 201707010291), China Postdoctoral Science Foundation funded project(2019M652845). Projects of Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Projects of Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Leading Specialist Construction Project‐Department of Urology, the First Affiliated Hospital, Jinan University(711006). Funding Information: This study was supported by grants from Guangzhou Municipal Science and Technology Project (201803040001), National Natural Science Foundation of China (81802567, 81802555, 815714278160062081470983), the Fundamental Research Funds for the Central Universities(21618306), Guangzhou Municipal Science and Technology Project (201803040001, 201710010081, 201707010291), China Postdoctoral Science Foundation funded project(2019M652845). Projects of Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Projects of Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Leading Specialist Construction Project-Department of Urology, the First Affiliated Hospital, Jinan University(711006). Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals, Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = aug,
day = "1",
doi = "10.1002/mc.23201",
language = "English (US)",
volume = "59",
pages = "897--907",
journal = "Molecular Carcinogenesis",
issn = "0899-1987",
publisher = "Wiley",
number = "8",
}