Application of physicochemically modified silicon substrates as reverse-phase protein microarrays

A. Jasper Nijdam, Michael R. Zianni, Edward E. Herderick, Mark M.C. Cheng, Jenifer R. Prosperi, Fredika A. Robertson, Emanuel F. Petricoin, Lance A. Liotta, Mauro Ferrari

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Physicochemically modified silicon substrates can provide a high quality alternative to nitrocellulose-oated glass slides for use in reverse-phase protein microarrays. Enhancement of protein microarray sensitivities is an important goal, especially because molecular targets within patient tissues exist in low abundance. The ideal array substrate has a high protein binding affinity and low intrinsic background signal. Silicon, which has low intrinsic autofluorescence, is being explored as a potential microarray surface. In a previous paper (Nijdam, A. J.; Cheng, M. M.-C.; Fedele, R.; Geho, D. H.; Herrmann, P.; Killian, K.; Espina, V.; Petricoin, E. F.; Liotta, L. A.; Ferrari, M. Physicochemically Modified Silicon as Substrate for Protein Microarrays. Biomaterials 2007, 28, 550-558), it is shown that physicochemical modification of silicon substrates increases the binding of protein to silicon to a level comparable with that of nitrocellulose. Here, we apply such substrates in a reverse-phase protein microarray setting in two model systems.

Original languageEnglish (US)
Pages (from-to)1247-1254
Number of pages8
JournalJournal of Proteome Research
Issue number3
StatePublished - Mar 6 2009
Externally publishedYes


  • Nanotechnology
  • Protein microarrays
  • Proteomics
  • Silicon

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)


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