Application of Laser Self-Beat Spectroscopic Technique to the Study of Solutions of Human Plasma Low-Density Lipoproteins

R. W. DeBlois, E. E. Uzgiris, S. K. Devi, Antonio Gotto

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The technique of self-beat or homodyne spectroscopy has been applied to the determination of the translational diffusion constant of human plasma low-density lipoproteins (LDL). Both power spectrum and autocorrelation methods give equivalent results, but with conventional equipment the power spectrum measurement takes on the order of hours while autocorrelation is accomplished in minutes. The effects of concentration of LDL, scattering angle of the light pH of the solution and buffer concentration were investigated. The diffusion constant obtained, D25,w = 2.14 ± 0.09 × 10-7 cm2/sec, was in good general accord with conventional measures. The equivalent spherical diameter, obtained from the Stokes-Einstein relationship, 229 ± 10 Å was within a broad band of values given by other techniques.

Original languageEnglish (US)
Pages (from-to)2645-2649
Number of pages5
JournalBiochemistry
Volume12
Issue number14
DOIs
StatePublished - Jul 1 1973

ASJC Scopus subject areas

  • Biochemistry

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